| RRC ID |
38815
|
| Author |
Shin SY, Shimizu M, Ohtaki T, Munekata E.
|
| Title |
Synthesis and biological activity of N-terminal-truncated derivatives of human epidermal growth factor (h-EGF).
|
| Journal |
Peptides
|
| Abstract |
To investigate the contribution of the N-terminal sequence of h-EGF to its biological activity and the formation of three intramolecular disulfide bonds by oxidative refolding via air oxidation, five derivatives of h-EGF with a single N-terminal amino acid deletion were synthesized by solid-phase synthesis. The homogeneity of the synthetic peptides was confirmed by analytical reversed-phase HPLC, amino acid analysis, and FAB-MS. The pairing of the three disulfide bridges in synthetic peptides was determined by thermolytic digestion. All N-truncated derivatives of h-FGF formed the correct intramolecular three disulfide linkages during oxidative refolding and had equipotent activity in both EGF receptor binding on A-431 epidermoid carcinoma cells and mitogenesis on NIH-3T3 fibroblast cells, compared with authentic h-EGF. The results suggested that the five residues from N-terminal sequence of h-EGF have no effect on the formation of the correct disulfide linkages in h-EGF and do not exert a significant influence on its biological activity.
|
| Volume |
16(2)
|
| Pages |
205-10
|
| Published |
1995-1-1
|
| DOI |
10.1016/0196-9781(94)00181-2
|
| PII |
0196-9781(94)00181-2
|
| PMID |
7784250
|
| MeSH |
3T3 Cells
Amino Acid Sequence
Animals
Binding, Competitive
Carcinoma, Squamous Cell
Cell Division / drug effects
Chromatography, High Pressure Liquid
Cysteine
Dose-Response Relationship, Drug
Epidermal Growth Factor / chemical synthesis*
Epidermal Growth Factor / chemistry
Epidermal Growth Factor / pharmacology*
ErbB Receptors / metabolism*
Humans
Indicators and Reagents
Kinetics
Mice
Molecular Sequence Data
Peptide Fragments / chemical synthesis*
Peptide Fragments / chemistry
Peptide Fragments / pharmacology*
Protein Conformation
Protein Folding
Tumor Cells, Cultured
|
| IF |
2.843
|
| Times Cited |
7
|
|
WOS Category
|
PHARMACOLOGY & PHARMACY
BIOCHEMISTRY & MOLECULAR BIOLOGY
ENDOCRINOLOGY & METABOLISM
|
| Resource |
| Human and Animal Cells |
A431(RCB0202) |
