RRC ID 38816
著者 Takenouchi T, Kadosaka M, Shin SY, Munekata E.
タイトル Chemical synthesis and biological activities of the EGF-like domain of mouse schwannoma-derived growth factor (SDGF).
ジャーナル Int J Pept Protein Res
Abstract Schwannoma-derived growth factor (SDGF), an epidermal growth factor (EGF) family peptide recently discovered, has an EGF-like domain in the carboxyl terminal portion. In this study, we synthesized mSDGF(38-80) corresponding to the EGF-like domain of mouse SDGF by means of stepwise solid-phase method using Fmoc chemistry in order to evaluate the biological function of the EGF-like domain in SDGF. The linear peptide of mSDGF(38-80) was folded by direct oxidation with reduced and oxidized glutathione to form intramolecular disulfide bridges in synthetic peptide. On the biological activity, we examined mitogenic activity induced by mSDGF(38-80) in NIH/3T3 fibroblast cells and interaction with EGF receptor in A431 cells. In the results, mSDGF(38-80) was confirmed to form three disulfide linkages that were similar in pattern to EGF by amino acid and sequence analysis of fragments obtained after thermolytic digestion. However, mSDGF(38-80) possessed weak mitogenic activity in NIH/3T3 cells and weak binding affinity for the EGF receptor in A431 cells compared with those of human EGF. These results suggest that the EGF-like domain of SDGF may have little effect upon mitogenic activity and the EGF receptor binding of SDGF.
巻・号 46(5)
ページ 391-6
公開日 1995-11-1
DOI 10.1111/j.1399-3011.1995.tb01073.x
PMID 8567183
MeSH 3T3 Cells Amino Acid Sequence Amphiregulin Animals Cell Division / drug effects Chromatography, High Pressure Liquid Disulfides / chemistry EGF Family of Proteins Epidermal Growth Factor / chemistry* ErbB Receptors / metabolism Glycoproteins* Growth Substances / chemistry* Humans Intercellular Signaling Peptides and Proteins* Mice Molecular Sequence Data Neurilemmoma / chemistry* Oxidation-Reduction Peptide Fragments / chemical synthesis* Peptide Fragments / metabolism Peptide Fragments / pharmacology* Protein Folding Sequence Analysis Spectrometry, Mass, Fast Atom Bombardment Structure-Activity Relationship Thermolysin / metabolism
引用数 2
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 A431(RCB0202)