RRC ID |
38816
|
Author |
Takenouchi T, Kadosaka M, Shin SY, Munekata E.
|
Title |
Chemical synthesis and biological activities of the EGF-like domain of mouse schwannoma-derived growth factor (SDGF).
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Journal |
Int J Pept Protein Res
|
Abstract |
Schwannoma-derived growth factor (SDGF), an epidermal growth factor (EGF) family peptide recently discovered, has an EGF-like domain in the carboxyl terminal portion. In this study, we synthesized mSDGF(38-80) corresponding to the EGF-like domain of mouse SDGF by means of stepwise solid-phase method using Fmoc chemistry in order to evaluate the biological function of the EGF-like domain in SDGF. The linear peptide of mSDGF(38-80) was folded by direct oxidation with reduced and oxidized glutathione to form intramolecular disulfide bridges in synthetic peptide. On the biological activity, we examined mitogenic activity induced by mSDGF(38-80) in NIH/3T3 fibroblast cells and interaction with EGF receptor in A431 cells. In the results, mSDGF(38-80) was confirmed to form three disulfide linkages that were similar in pattern to EGF by amino acid and sequence analysis of fragments obtained after thermolytic digestion. However, mSDGF(38-80) possessed weak mitogenic activity in NIH/3T3 cells and weak binding affinity for the EGF receptor in A431 cells compared with those of human EGF. These results suggest that the EGF-like domain of SDGF may have little effect upon mitogenic activity and the EGF receptor binding of SDGF.
|
Volume |
46(5)
|
Pages |
391-6
|
Published |
1995-11-1
|
DOI |
10.1111/j.1399-3011.1995.tb01073.x
|
PMID |
8567183
|
MeSH |
3T3 Cells
Amino Acid Sequence
Amphiregulin
Animals
Cell Division / drug effects
Chromatography, High Pressure Liquid
Disulfides / chemistry
EGF Family of Proteins
Epidermal Growth Factor / chemistry*
ErbB Receptors / metabolism
Glycoproteins*
Growth Substances / chemistry*
Humans
Intercellular Signaling Peptides and Proteins*
Mice
Molecular Sequence Data
Neurilemmoma / chemistry*
Oxidation-Reduction
Peptide Fragments / chemical synthesis*
Peptide Fragments / metabolism
Peptide Fragments / pharmacology*
Protein Folding
Sequence Analysis
Spectrometry, Mass, Fast Atom Bombardment
Structure-Activity Relationship
Thermolysin / metabolism
|
Times Cited |
2
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
Resource |
Human and Animal Cells |
A431(RCB0202) |