Reference - Detail
| RRC ID | 38817 |
|---|---|
| Author | Takenouchi T, Munekata E. |
| Title | beta-Amyloid peptide, substance P, and SEC receptor ligand activate cytoplasmic Ca2+ in neutrophil-like HL-60 cells: effect of chemotactic peptide antagonist BocMLF. |
| Journal | Peptides |
| Abstract |
It has been reported that a discrete peptide fragment of beta-amyloid protein, beta A(25-35), and neuropeptide substance P (SP) possessed sequence homology and could bind to the serine protease inhibitor (serpin) enzyme complex (SEC) receptor. Thus, it has been thought that these peptides and SEC receptor ligand might have similar biological activities. In the present study, we found that C-terminal amidated beta A(25-35)-NH2, SP, and the SEC receptor ligand, Phe-Val-Phe-Leu-Met(FVFLM), could induce an increase in the intracellular free Ca2+ concentration ([Ca2+]i) in neutrophil-like human leukemic (HL-60) cells. Pretreatment with pertussis toxin (PTX) potently inhibited the increase in [Ca2+]i stimulated by these peptides, suggesting that these responses might be mediated by PTX-sensitive G-proteins. Furthermore, we examined the effect on these responses of t-butyloxycarbonyl-methionyl-leucyl-phenylalanine (BocMLF), which is a competitive antagonist of chemotactic peptide N-formyl-methionyl-leucyl-phenylalanine (fMLF) at its receptor. BocMLF scarcely inhibited the [Ca2+]i increase stimulated by beta A(25-35)-NH2. However, the increase in FVFLM-induced [Ca2+]i was potently inhibited by BocMLF. The results suggest that the [Ca2+]i activation of beta A(25-35)-NH2 may have a different mechanism from that of FVFLM in neutrophil-like HL-60 cells, which is not mediated by the SEC-receptor. |
| Volume | 16(6) |
| Pages | 1019-24 |
| Published | 1995-1-1 |
| DOI | 10.1016/0196-9781(95)00084-w |
| PII | 0196-9781(95)00084-W |
| PMID | 8532582 |
| MeSH | Amino Acid Sequence Amyloid beta-Peptides / chemistry Amyloid beta-Peptides / pharmacology Calcium / metabolism* Cell Line Cytoplasm / metabolism Humans Ligands Molecular Sequence Data N-Formylmethionine Leucyl-Phenylalanine / antagonists & inhibitors* Neutrophils / drug effects* Neutrophils / metabolism* Oligopeptides / chemistry Oligopeptides / pharmacology* Peptide Fragments / chemistry Peptide Fragments / pharmacology Receptors, Cell Surface / metabolism Substance P / pharmacology |
| IF | 2.843 |
| Times Cited | 16 |
| WOS Category | PHARMACOLOGY & PHARMACY ENDOCRINOLOGY & METABOLISM BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 4 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | HL60(RCB0041) |