| RRC ID |
3881
|
| Author |
Tomita M, Morohoshi F, Matsumoto Y, Otsuka K, Sakai K.
|
| Title |
Role of DNA double-strand break repair genes in cell proliferation under low dose-rate irradiation conditions.
|
| Journal |
J Radiat Res
|
| Abstract |
Radiation-induced DNA double-stand breaks (DSBs) lead to numerous biological effects. To elucidate the molecular mechanisms involved in cellular responses to low dose and low dose-rate radiation, it is informative to clarify the roles of DSB repair related genes. In higher vertebrate cells, there are at least two major DSB repair pathways, namely non-homologous end-joining (NHEJ) and homologous recombination (HR). Here, it is shown that in chicken DT40 cells irradiated with gamma-rays at a low dose-rate (2.4 cGy/day), the growth delay in NHEJ-related KU70- and PRKDC (encoding DNA-PKcs)-defective cells were remarkably higher than in cells defective for the HR-related RAD51B and RAD54 genes. DNA-PKcs- defective human M059J cells also showed an obvious growth delay when compared to control M059K cells. RAD54(-/-)KU70(-/-) cells demonstrated their highest degree of growth delay after an X-irradiation with a high dose-rate of 0.9 Gy/min. However they showed a lower degree of growth delay than that seen in KU70(-/-) and PRKDC(-/-/-) cells exposed to low dose-rate irradiation. These findings indicate that cellular responses to low dose-rate radiation are remarkably different from those to high dose-rate radiation. The fact that both DT40 and mammalian NHEJ-defective cells were highly sensitive to low dose-rate radiation, provide a foundation for the concept that NHEJ-related factors may be useful as molecular markers to predict the sensitivity of humans to low dose-rate radiation.
|
| Volume |
49(5)
|
| Pages |
557-64
|
| Published |
2008-9-1
|
| DOI |
10.1269/jrr.08036
|
| PII |
JST.JSTAGE/jrr/08036
|
| PMID |
18797158
|
| MeSH |
Animals
B-Lymphocytes / physiology*
B-Lymphocytes / radiation effects*
Cell Line
Cell Proliferation / radiation effects*
Chickens
DNA / genetics*
DNA / radiation effects*
DNA Damage*
DNA Repair / physiology*
DNA Repair / radiation effects*
Dose-Response Relationship, Radiation
Radiation Dosage
|
| IF |
1.95
|
| Times Cited |
18
|
|
WOS Category
|
BIOLOGY
RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
|
| Resource |
| Human and Animal Cells |
DT40(RCB1464)
ΔKu70-DT40(RCB1621)
ΔRad54-DT40(RCB162()
ΔRad51B-DT40(RCB1622)
ΔKu70ΔRad54B-DT40(RCB1632) |
