| RRC ID |
38824
|
| 著者 |
Matsuki H, Fujimoto N, Iwata K, Knäuper V, Okada Y, Hayakawa T.
|
| タイトル |
A one-step sandwich enzyme immunoassay for human matrix metalloproteinase 8 (neutrophil collagenase) using monoclonal antibodies.
|
| ジャーナル |
Clin Chim Acta
|
| Abstract |
A one-step sandwich enzyme immunoassay (EIA) system for human matrix metalloproteinase 8 (MMP-8, neutrophil collagenase, EC 3.4.24.7) has been established with a pair of monoclonal antibodies prepared against the zymogen of MMP-8 purified from human neutrophils. MMP-8 in samples simultaneously reacted with both solid-phase and peroxidase-labeled antibodies. Sensitivity of this EIA system was 0.34 micrograms/l (5.7 pg/assay) and linearity was obtained between 0.5 and 500 micrograms/l (8.3-8300 pg/assay). The EIA system recognized both precursor and active forms of MMP-8 but not MMP-8 complexed with tissue inhibitors of metalloproteinases. There was no difference in the MMP-8 levels between the plasma samples from patients with rheumatoid arthritis or osteoarthritis and those from healthy subjects (median 6.2 micrograms/l, range 1.5-28 micrograms/l). However, the level in synovial fluids from patients with rheumatoid arthritis (median 345 micrograms/l, range 84-2860 micrograms/l) was shown to be higher than that from osteoarthritic patients. MMP-8 levels in human whole saliva from patients with periodontal diseases (median 282 micrograms/l, range 0-1420 micrograms/l) were also significantly higher than those from clinically healthy subjects (median 25 micrograms/l, range 0-100 micrograms/l). Immunoreactivity analyses showed that MMP-8 species in normal human plasma exists as a precursor but not as a complex form with tissue inhibitor of metalloproteinases (TIMP)-1 or TIMP-2.
|
| 巻・号 |
244(2)
|
| ページ |
129-43
|
| 公開日 |
1996-1-31
|
| DOI |
10.1016/0009-8981(95)06197-5
|
| PII |
0009898195061975
|
| PMID |
8714431
|
| MeSH |
Animals
Antibodies, Monoclonal
Antibody Specificity
Arthritis, Rheumatoid / blood
Arthritis, Rheumatoid / enzymology
Biomarkers
Cell Line
Chromatography, Affinity
Chromatography, Gel
Collagenases / analysis*
Collagenases / blood
Collagenases / isolation & purification
Electrophoresis, Polyacrylamide Gel
Female
Humans
Immunoenzyme Techniques
Immunohistochemistry
Matrix Metalloproteinase 8
Mice
Mice, Inbred BALB C
Molecular Weight
Neutrophils / enzymology
Osteoarthritis / blood
Osteoarthritis / enzymology
Placenta / enzymology
Pregnancy
Saliva / enzymology
Statistics, Nonparametric
Stomach Neoplasms / enzymology
Stomach Neoplasms / pathology
Stomach Neoplasms / surgery
Synovial Fluid / enzymology
Tumor Cells, Cultured
|
| IF |
2.615
|
| 引用数 |
40
|
|
WOS 分野
|
MEDICAL LABORATORY TECHNOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
NB1RGB(RCB0222) |
