論文 - 詳細
| RRC ID | 38824 |
|---|---|
| 著者 | Matsuki H, Fujimoto N, Iwata K, Knäuper V, Okada Y, Hayakawa T. |
| タイトル | A one-step sandwich enzyme immunoassay for human matrix metalloproteinase 8 (neutrophil collagenase) using monoclonal antibodies. |
| ジャーナル | Clin Chim Acta |
| Abstract |
A one-step sandwich enzyme immunoassay (EIA) system for human matrix metalloproteinase 8 (MMP-8, neutrophil collagenase, EC 3.4.24.7) has been established with a pair of monoclonal antibodies prepared against the zymogen of MMP-8 purified from human neutrophils. MMP-8 in samples simultaneously reacted with both solid-phase and peroxidase-labeled antibodies. Sensitivity of this EIA system was 0.34 micrograms/l (5.7 pg/assay) and linearity was obtained between 0.5 and 500 micrograms/l (8.3-8300 pg/assay). The EIA system recognized both precursor and active forms of MMP-8 but not MMP-8 complexed with tissue inhibitors of metalloproteinases. There was no difference in the MMP-8 levels between the plasma samples from patients with rheumatoid arthritis or osteoarthritis and those from healthy subjects (median 6.2 micrograms/l, range 1.5-28 micrograms/l). However, the level in synovial fluids from patients with rheumatoid arthritis (median 345 micrograms/l, range 84-2860 micrograms/l) was shown to be higher than that from osteoarthritic patients. MMP-8 levels in human whole saliva from patients with periodontal diseases (median 282 micrograms/l, range 0-1420 micrograms/l) were also significantly higher than those from clinically healthy subjects (median 25 micrograms/l, range 0-100 micrograms/l). Immunoreactivity analyses showed that MMP-8 species in normal human plasma exists as a precursor but not as a complex form with tissue inhibitor of metalloproteinases (TIMP)-1 or TIMP-2. |
| 巻・号 | 244(2) |
| ページ | 129-43 |
| 公開日 | 1996-1-31 |
| DOI | 10.1016/0009-8981(95)06197-5 |
| PII | 0009898195061975 |
| PMID | 8714431 |
| MeSH | Animals Antibodies, Monoclonal Antibody Specificity Arthritis, Rheumatoid / blood Arthritis, Rheumatoid / enzymology Biomarkers Cell Line Chromatography, Affinity Chromatography, Gel Collagenases / analysis* Collagenases / blood Collagenases / isolation & purification Electrophoresis, Polyacrylamide Gel Female Humans Immunoenzyme Techniques Immunohistochemistry Matrix Metalloproteinase 8 Mice Mice, Inbred BALB C Molecular Weight Neutrophils / enzymology Osteoarthritis / blood Osteoarthritis / enzymology Placenta / enzymology Pregnancy Saliva / enzymology Statistics, Nonparametric Stomach Neoplasms / enzymology Stomach Neoplasms / pathology Stomach Neoplasms / surgery Synovial Fluid / enzymology Tumor Cells, Cultured |
| IF | 2.615 |
| 引用数 | 40 |
| WOS 分野 | MEDICAL LABORATORY TECHNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | NB1RGB(RCB0222) |