RRC ID 38830
Author Shirasawa Y, Sato S, Osawa T, Hirashima A.
Title Transdominant inhibition of Moloney murine leukemia virus proliferation by defective mutants of reverse transcriptase.
Journal J. Biochem.
Abstract To investigate the dominant-negative inhibition of Moloney murine leukemia virus (Mo-MuLV) proliferation by polymerization-defective mutants of reverse transcriptase (RT), we constructed several plasmids harboring the Mo-MuLV RT gene in which the YVDD sequence, one of the conserved sequences in RNA-dependent polymerases, was altered, and then transformed mouse NIH3T3 cells and Escherichia coli with the mutant plasmids. Mouse NIH3T3 cells expressing these mutant RT genes were highly resistant to Mo-MuLV proliferation. The mutant RT expressed in E. coli exhibited no polymerization activity, but it retained its binding activity to the template RNA and inhibited in vitro poly(dG) synthesis occurring with the wild-type RT. These results suggest that the competition for binding of the two types of enzymes to the template is responsible for the resistance to Mo-MuLV proliferation and that the YVDD sequence of Mo-MuLV may be a good target for dominant-negative inhibition of retroviral proliferation.
Volume 119(6)
Pages 1070-5
Published 1996-6
DOI 10.1093/oxfordjournals.jbchem.a021349
PMID 8827439
MeSH 3T3 Cells / metabolism 3T3 Cells / virology Amino Acid Sequence Animals Conserved Sequence DNA, Complementary / antagonists & inhibitors DNA, Complementary / biosynthesis Escherichia coli / genetics Escherichia coli / metabolism Genes, Viral / genetics Mice Moloney murine leukemia virus / enzymology* Moloney murine leukemia virus / genetics Moloney murine leukemia virus / physiology Mutation Plasmids / genetics Poly A / metabolism Poly G / antagonists & inhibitors Poly G / biosynthesis Protein Binding RNA-Directed DNA Polymerase / genetics RNA-Directed DNA Polymerase / metabolism* Transfection Viral Proteins / genetics Viral Proteins / metabolism Virus Replication
IF 2.23
Times Cited 0
Human and Animal Cells