論文 - 詳細
| RRC ID | 38835 |
|---|---|
| 著者 | Hikawa N, Takenaka T. |
| タイトル | Improved method for producing neuronal hybrids using emetine and actinomycin D. |
| ジャーナル | Brain Res |
| Abstract |
We modified the method of somatic cell fusion for neurons to improve the efficiency of hybrid production. C1300 neuroblastoma cells were incubated with emetine and actinomycin D before fusion. After fusion between C1300 cells and adult mouse dorsal root ganglia neurons with polyethyleneglycol, we were able to select the hybrids from non-fused cells. We obtained hybrid clones at high efficiency (7.2 clones/10(4) neurons). |
| 巻・号 | 734(1-2) |
| ページ | 345-8 |
| 公開日 | 1996-9-23 |
| PII | 0006-8993(96)00888-8 |
| PMID | 8896846 |
| MeSH | Animals Cell Fusion* Cytological Techniques* Dactinomycin / pharmacology* Emetine / pharmacology* Ganglia, Spinal / cytology Hybrid Cells Mice Mice, Inbred C57BL Neurons / drug effects* Neurons / physiology* Nucleic Acid Synthesis Inhibitors / pharmacology Tumor Cells, Cultured |
| IF | 2.733 |
| 引用数 | 5 |
| WOS 分野 | NEUROSCIENCES |
| リソース情報 | |
| ヒト・動物細胞 | C-1300(RCB0283) |