Reference - Detail
| RRC ID | 38837 |
|---|---|
| Author | Okada N, Fushimi M, Nagata Y, Fukunaga T, Tsutsumi Y, Nakagawa S, Mayumi T. |
| Title | Evaluation of angiogenic inhibitors with an in vivo quantitative angiogenesis method using agarose microencapsulation and mouse hemoglobin enzyme-linked immunosorbent assay. |
| Journal | Jpn J Cancer Res |
| Abstract |
In the present work, using a previously reported in vivo quantitative tumor-angiogenesis model, we attempted to ascertain whether this animal model is suitable for practical use in monitoring inhibitors of tumor angiogenesis. Mouse sarcoma-180 cells, human A431 cells or rat C6 cells microencapsulated in agarose beads were implanted s.c. into C57BL/6 mice. The level of blood vessel induction at the agarose pellet site was evaluated using mouse hemoglobin enzyme-linked immunosorbent assay on day 10 after implantation. Hydrocortisone, tetrahydro-S, medroxyprogesterone acetate, pentosan polysulfate and suramin inhibited blood vessel growth in our in vivo tumor-angiogenesis assay system, and heparin enhanced the antiangiogenic effects of hydrocortisone and tetrahydro-S. These results are almost entirely consistent with those observed in common assay systems, and suggest that this method may be useful for the identification and quantitative evaluation of inhibitors of tumor angiogenesis. |
| Volume | 87(9) |
| Pages | 952-7 |
| Published | 1996-9-1 |
| DOI | 10.1111/j.1349-7006.1996.tb02125.x |
| PII | 0910505096859023 |
| PMID | 8878458 |
| PMC | PMC5921206 |
| MeSH | Animals Carcinoma, Squamous Cell / blood supply* Carcinoma, Squamous Cell / drug therapy* Cortodoxone / analogs & derivatives Cortodoxone / pharmacology Drug Compounding Drug Evaluation, Preclinical Enzyme-Linked Immunosorbent Assay Female Hemoglobins* Humans Hydrocortisone / pharmacology Male Medroxyprogesterone / pharmacology Mice Mice, Inbred C57BL Neovascularization, Pathologic / drug therapy* Pentosan Sulfuric Polyester / pharmacology Sarcoma 180 / blood supply* Sarcoma 180 / drug therapy* Sepharose* Suramin / pharmacology Tumor Cells, Cultured |
| Times Cited | 10 |
| WOS Category | ONCOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Resource | |
| Human and Animal Cells | A431(RCB0202) HL60(RCB0041) |