| RRC ID |
38849
|
| 著者 |
Yamada H, Iwase S, Nagai M, Nemoto T, Sekikawa T, Takahara S, Nakada S, Furukawa Y, Horiguchi-Yamada J.
|
| タイトル |
Herbimycin A down-regulates messages of cyclin D1 and c-myc during erythroid differentiation of K562 cells.
|
| ジャーナル |
Int J Hematol
|
| Abstract |
The ansamycin antibiotic, herbimycin A, is a potent tyrosine kinase inhibitor, and induces the erythroid differentiation of bcr-abl-possessing K562 cells. The growth of K562 cells was cytostatically reduced to less than 50% of the control level at 48 h by 0.5 microgram/ml of herbimycin A treatment. A total of 12% and 53% of the treated cells were benzidine-positive at 24 h and 48 h, respectively. The percentage of cells in the S phase decreased rapidly from 60% to 15% after 12 h of treatment. The reduction of S phase cells persisted until 24 h, whereas the G1 population conversely increased. Then underphosphorylated retinoblastoma gene product increased from 6 h to 24 h, but returned to baseline at 48 h. Most cell cycle controlling genes were unchanged by herbimycin A treatment. However, both cyclin D1 and c-myc were prominently down-regulated in the early phase of treatment, corresponding to the decline of the S phase population. Cyclin D1 was initially down-regulated to an undetectable level at 6 h, although its expression recovered gradually from 12 h and returned to baseline at 24 h. c-myc was also down-regulated from 1 h to 6 h. These data suggest that signals originating from bcr-abl kinase are at least partly transduced through both c-myc and cyclin D1, and that herbimycin A-induced erythroid differentiation occurs during or after the cessation of growth due to interference with these signals.
|
| 巻・号 |
65(1)
|
| ページ |
31-40
|
| 公開日 |
1996-12-1
|
| DOI |
10.1016/s0925-5710(96)00526-9
|
| PII |
S0925571096005269
|
| PMID |
8990623
|
| MeSH |
Antibiotics, Antineoplastic / pharmacology*
Benzoquinones
Cell Differentiation / drug effects
Cyclin D1
Cyclins / genetics*
Down-Regulation
Erythroid Precursor Cells / cytology
Erythroid Precursor Cells / drug effects*
Humans
Lactams, Macrocyclic
Neoplasm Proteins / genetics*
Oncogene Proteins / genetics*
Proto-Oncogene Proteins c-myc / genetics
Quinones / pharmacology*
RNA, Messenger / drug effects*
Rifabutin / analogs & derivatives
Tumor Cells, Cultured
|
| IF |
2.245
|
| 引用数 |
14
|
|
WOS 分野
|
HEMATOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
K562(RCB0027) |
