論文 - 詳細
| RRC ID | 38849 |
|---|---|
| 著者 | Yamada H, Iwase S, Nagai M, Nemoto T, Sekikawa T, Takahara S, Nakada S, Furukawa Y, Horiguchi-Yamada J. |
| タイトル | Herbimycin A down-regulates messages of cyclin D1 and c-myc during erythroid differentiation of K562 cells. |
| ジャーナル | Int J Hematol |
| Abstract |
The ansamycin antibiotic, herbimycin A, is a potent tyrosine kinase inhibitor, and induces the erythroid differentiation of bcr-abl-possessing K562 cells. The growth of K562 cells was cytostatically reduced to less than 50% of the control level at 48 h by 0.5 microgram/ml of herbimycin A treatment. A total of 12% and 53% of the treated cells were benzidine-positive at 24 h and 48 h, respectively. The percentage of cells in the S phase decreased rapidly from 60% to 15% after 12 h of treatment. The reduction of S phase cells persisted until 24 h, whereas the G1 population conversely increased. Then underphosphorylated retinoblastoma gene product increased from 6 h to 24 h, but returned to baseline at 48 h. Most cell cycle controlling genes were unchanged by herbimycin A treatment. However, both cyclin D1 and c-myc were prominently down-regulated in the early phase of treatment, corresponding to the decline of the S phase population. Cyclin D1 was initially down-regulated to an undetectable level at 6 h, although its expression recovered gradually from 12 h and returned to baseline at 24 h. c-myc was also down-regulated from 1 h to 6 h. These data suggest that signals originating from bcr-abl kinase are at least partly transduced through both c-myc and cyclin D1, and that herbimycin A-induced erythroid differentiation occurs during or after the cessation of growth due to interference with these signals. |
| 巻・号 | 65(1) |
| ページ | 31-40 |
| 公開日 | 1996-12-1 |
| DOI | 10.1016/s0925-5710(96)00526-9 |
| PII | S0925571096005269 |
| PMID | 8990623 |
| MeSH | Antibiotics, Antineoplastic / pharmacology* Benzoquinones Cell Differentiation / drug effects Cyclin D1 Cyclins / genetics* Down-Regulation Erythroid Precursor Cells / cytology Erythroid Precursor Cells / drug effects* Humans Lactams, Macrocyclic Neoplasm Proteins / genetics* Oncogene Proteins / genetics* Proto-Oncogene Proteins c-myc / genetics Quinones / pharmacology* RNA, Messenger / drug effects* Rifabutin / analogs & derivatives Tumor Cells, Cultured |
| IF | 2.245 |
| 引用数 | 14 |
| WOS 分野 | HEMATOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | K562(RCB0027) |