論文 - 詳細
| RRC ID | 38853 |
|---|---|
| 著者 | Wang DS, Yamazaki K, Nohtomi K, Shizume K, Ohsumi K, Shibuya M, Demura H, Sato K. |
| タイトル | Increase of vascular endothelial growth factor mRNA expression by 1,25-dihydroxyvitamin D3 in human osteoblast-like cells. |
| ジャーナル | J Bone Miner Res |
| Abstract |
Vascular endothelial growth factor (VEGF), a secreted endothelial cell-specific mitogen, is produced in endocrine organs and regulated by trophic hormones. Because angiogenesis and osteogenesis are closely regulated, we studied whether human osteoblast-like cells produce VEGF, and if so, what factors regulate VEGF mRNA expression. Human osteoblast-like cells (HObLC) derived from trabecular bone explants were cultured in alpha-MEM supplemented with 10% fetal calf serum. Northern blot analysis revealed that HObLC expressed VEGF mRNA, as did several human osteosarcoma cells. 1,25-(OH)2D3 increased the steady-state levels of VEGF mRNA in a time- and concentration-dependent manner in HObLC and one of the osteosarcoma cell lines, SaOS-2, accompanied by an increase in the concentration of immunoreactive VEGF in the conditioned medium. PTH and IGF-I also increased the level of VEGF mRNA in HObLC and SaOS-2 cells. Furthermore, 12-O-tetradecanoylphorbol ester stimulated VEGF mRNA in a time-and concentration-dependent manner. The VEGF mRNA expression induced by 1,25-(OH)2D3 was completely inhibited by H-7, but only partially by staurosporine. We have demonstrated that PTH, IGF-I, and most potently 1,25-(OH)2D3 stimulate the mRNA expression and secretion of VEGF in human osteoblast-like cells, suggesting that one of the anabolic effects of 1,25-(OH)2D3 on skeletal tissue may be mediated by VEGF produced by osteoblasts. |
| 巻・号 | 11(4) |
| ページ | 472-9 |
| 公開日 | 1996-4-1 |
| DOI | 10.1002/jbmr.5650110408 |
| PMID | 8992878 |
| MeSH | Autoradiography Blotting, Northern Bone Neoplasms / genetics Bone Neoplasms / pathology Calcitriol / pharmacology* Cells, Cultured Dose-Response Relationship, Drug Endothelial Growth Factors / genetics* Endothelial Growth Factors / metabolism Gene Expression Regulation, Neoplastic / drug effects Gene Expression Regulation, Neoplastic / genetics Glyceraldehyde-3-Phosphate Dehydrogenases / metabolism Humans Insulin-Like Growth Factor I / pharmacology Lymphokines / genetics* Lymphokines / metabolism Osteoblasts / cytology Osteoblasts / drug effects* Osteoblasts / metabolism Osteosarcoma / genetics Osteosarcoma / pathology Parathyroid Hormone / pharmacology RNA, Messenger / genetics RNA, Messenger / metabolism* Tumor Cells, Cultured Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factors |
| IF | 5.854 |
| 引用数 | 114 |
| WOS 分野 | ENDOCRINOLOGY & METABOLISM |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | OST(RCB0454) |