論文 - 詳細
| RRC ID | 38860 |
|---|---|
| 著者 | Ohuchi E, Azumano I, Yoshida S, Iwata K, Okada Y. |
| タイトル | A one-step sandwich enzyme immunoassay for human matrix metalloproteinase 7 (matrilysin) using monoclonal antibodies. |
| ジャーナル | Clin Chim Acta |
| Abstract |
A one-step sandwich enzyme immunoassay (EIA) system for human matrix metalloproteinase 7 (MMP-7, matrilysin, EC 3.4.24.23) was established with a pair of monoclonal antibodies prepared against the zymogen of MMP-7 (proMMP-7) purified from CaR-1 human rectal carcinoma cells and against an oligopeptide corresponding to the C-terminal domain of human proMMP-7. ProMMP-7 in samples was allowed to simultaneously react with both solid-phase and peroxidase-labeled antibodies. Sensitivity of the EIA system in the presence of EDTA was 0.05 microgram/l (1.5 pg/assay) and linearity was obtained between 0.156-10 micrograms/l (4.68-300 pg/assay). ProMMP-7 levels in human sera from healthy subjects were shown to be in the range of 10.7 +/- 18.8 micrograms/l. Gel filtration analyses indicated that proMMP-7 makes polymers after treatment with EDTA and suggested that proMMP-7 exists as polymers in normal human sera. |
| 巻・号 | 244(2) |
| ページ | 181-98 |
| 公開日 | 1996-1-31 |
| DOI | 10.1016/0009-8981(95)06199-1 |
| PII | 0009898195061991 |
| PMID | 8714436 |
| MeSH | Amino Acid Sequence Antibodies, Monoclonal Cell Line Chromatography, Gel Colonic Neoplasms / blood* Colonic Neoplasms / enzymology Colonic Neoplasms / pathology Electrophoresis, Polyacrylamide Gel Enzyme Precursors / blood Humans Immunoblotting Immunoenzyme Techniques Matrix Metalloproteinase 7 Metalloendopeptidases / blood* Molecular Sequence Data Molecular Weight Neoplasm Staging Peptide Fragments / chemical synthesis Peptide Fragments / immunology Rectal Neoplasms Reference Values Sensitivity and Specificity Tumor Cells, Cultured |
| IF | 2.615 |
| 引用数 | 31 |
| WOS 分野 | MEDICAL LABORATORY TECHNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | NB1RGB(RCB0222) |