| RRC ID |
38926
|
| 著者 |
Miyoshi Y, Date I, Ohmoto T, Iwata H.
|
| タイトル |
Histological analysis of microencapsulated dopamine-secreting cells in agarose/poly(styrene sulfonic acid) mixed gel xenotransplanted into the brain.
|
| ジャーナル |
Exp Neurol
|
| Abstract |
PC12 cells were encapsulated within agarose/poly-(styrene sulfonic acid) (agarose/PSSa) mixed gel and xenotransplanted into the brains of guinea pigs. The immunoprotection capability and biocompatibility of the agarose/PSSa microcapsules were evaluated. Tyrosine hydroxylase-positive PC12 cells within the microcapsules were observed in all guinea pigs at least 5 weeks after transplantation. PC12 cells had a relatively uniform small size and occasionally formed cell clusters; however, cell necrosis was not apparent. The host reaction to agarose/PSSa microcapsules was minimal. The degree of glial fibrillary acidic protein-positive astrocyte density around the microcapsules was similar to that around the injection tracks. Necrosis around the brain/microcapsule interface was not apparent as assessed by Nissl staining. Normal-appearing neurons were observed in close vicinity to the capsule. High-performance liquid chromatography with electrochemical detection showed basal and potassium-evoked release of dopamine from the PC12 cell-enclosed microcapsules in vitro. We conclude that encapsulation using agarose/PSSa mixed gel can not only isolate the enclosed cells from the host immune system but can also allow diffusional exchange of nutrients and neurotransmitters. Encapsulation using agarose/PSSa mixed gel may expand the range of donor tissues for neural transplantation.
|
| 巻・号 |
138(1)
|
| ページ |
169-75
|
| 公開日 |
1996-3-1
|
| DOI |
10.1006/exnr.1996.0055
|
| PII |
S0014-4886(96)90055-2
|
| PMID |
8593892
|
| MeSH |
Animals
Biocompatible Materials*
Corpus Striatum / cytology
Corpus Striatum / immunology
Dopamine / metabolism*
Female
Guinea Pigs
Neurons / transplantation*
PC12 Cells
Polymers
Rats
Sepharose
Sulfonic Acids
Transplantation, Heterologous
|
| IF |
4.691
|
| 引用数 |
29
|
|
WOS 分野
|
NEUROSCIENCES
|
| リソース情報 |
| ヒト・動物細胞 |
PC-12(RCB0009) |
