| RRC ID |
38946
|
| 著者 |
Shibata Y, Takiguchi H, Tamura K, Yamanaka K, Tezuka M, Abiko Y.
|
| タイトル |
Stimulation of interleukin-1beta-converting enzyme activity during growth inhibition by CPT-11 in the human myeloid leukemia cell line K562.
|
| ジャーナル |
Biochem Mol Med
|
| Abstract |
Camptothecin (CPT) was first extracted from Camptotheca acuminata and has a strong antitumor effect. Its water-soluble derivative, CPT-11, has higher therapeutic efficacy and less toxicity than CPT. Recently, CPT-treated cells have been shown to undergo apoptosis. However, the mechanism of induction of apoptosis by CPT has not been characterized in detail in any type of cells. On the other hand, interleukin-1beta-converting enzyme (ICE) is a mammalian homologue of CED3, a protein required for apoptosis in the nematode Caenorhabditis elegans. To determine how CPT-11 brings about cell death by apoptosis, we investigated the effects of CPT-11 on the expression of ICE activity in K562 cells, which represent human myeloid leukemia cells. The proliferation of K562 cells was shown to be inhibited by the presence of CPT-11 in the culture medium. We also found that the levels of mRNA for ICE in the cells were increased in the presence of CPT-11. Furthermore, we demonstrated that when CPT-11 was added to the culture medium, apoptosis of K562 cells was clearly detected in situ. These features suggested that CPT-11 enhances the apoptotic cell death in K562 cells and that a part of induction of apoptosis by CPT-11 may be correlated with the stimulation of the ICE activity.
|
| 巻・号 |
57(1)
|
| ページ |
25-30
|
| 公開日 |
1996-2-1
|
| DOI |
10.1006/bmme.1996.0005
|
| PII |
S1077315096900058
|
| PMID |
8812723
|
| MeSH |
Apoptosis
Base Sequence
Camptothecin / analogs & derivatives*
Camptothecin / pharmacology
Caspase 1
Cysteine Endopeptidases / biosynthesis*
Gene Expression Regulation, Leukemic
Humans
In Situ Hybridization
Irinotecan
Leukemia, Myeloid / drug therapy*
Leukemia, Myeloid / enzymology*
Molecular Sequence Data
Polymerase Chain Reaction
RNA, Messenger / metabolism
Tumor Cells, Cultured
|
| 引用数 |
3
|
|
WOS 分野
|
MEDICINE, RESEARCH & EXPERIMENTAL
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
K562(RCB0027) |
