論文 - 詳細
| RRC ID | 38958 |
|---|---|
| 著者 | Yoshiko Y, Kosugi T, Koide Y. |
| タイトル | Effects of a synthetic N-terminal fragment of stanniocalcin on the metabolism of mammalian bone in vitro. |
| ジャーナル | Biochim Biophys Acta |
| Abstract |
A synthetic peptide corresponding to the N-terminal amino acid residues of stanniocalcin (STC1-20) and including a region that is known to be an active site in teleosts was prepared and tested for its effects on the metabolism of mammalian bone in vitro. STC1-20 (10(-10)-10(-12) M) inhibited increases in the number of tartrate-resistant acid phosphatase-positive, multinucleated cells promoted by an N-terminal fragment of human parathyroid hormone (hPTH1-34) in cultures of murine hemopoietic cells. STC1-20 also slightly decreased the rate of loss of radioactivity from calvariae of fetal rats that had been prelabeled with 45Ca, both with and without stimulation by hPTH1-34. The accumulation of cAMP induced by hPTH1-34 in ROS 17/2.8-5 cells was suppressed by STC1-20 (10(-10)-10(-12) M). Treatment with STC1-20 (10(-11)-10(-13) M) caused increases of the rate of incorporation of [3H]proline into the collagenase-digestible protein of calvariae in newborn mice. From these results, it appears that STC1-20 has diverse effects on the metabolism of mammalian bone, causing a biphasic response. Such effects have not been observed with intact stanniocalcin or with materials from the corpuscles of Stannius and they are also different from the effects of hPTH1-34. |
| 巻・号 | 1311(3) |
| ページ | 143-9 |
| 公開日 | 1996-5-28 |
| DOI | 10.1016/0167-4889(95)00160-3 |
| PII | 0167-4889(95)00160-3 |
| PMID | 8664340 |
| MeSH | Acid Phosphatase / metabolism Amino Acid Sequence Animals Binding Sites Bone and Bones / drug effects Bone and Bones / metabolism* Calcium / metabolism* Cell Count / drug effects Cyclic AMP / metabolism Female Glycoproteins / chemistry Glycoproteins / pharmacology* Hormones / chemistry Hormones / pharmacology* Humans Isoenzymes / metabolism Mice Molecular Sequence Data Parathyroid Hormone / pharmacology Peptide Fragments / chemical synthesis Peptide Fragments / pharmacology* Rats Rats, Sprague-Dawley Salmon Sequence Homology, Amino Acid Skull Tartrate-Resistant Acid Phosphatase Teriparatide Tumor Cells, Cultured |
| IF | 3.411 |
| 引用数 | 12 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | ROS-17/2.8-5(RCB0462) |