| RRC ID |
38971
|
| 著者 |
Murata-Kamiya N, Kamiya H, Kaji H, Kasai H.
|
| タイトル |
Glyoxal, a major product of DNA oxidation, induces mutations at G:C sites on a shuttle vector plasmid replicated in mammalian cells.
|
| ジャーナル |
Nucleic Acids Res
|
| Abstract |
Glyoxal is a major product of DNA oxidation in which Fenton-type oxygen free radical-forming systems are involved. To determine the mutation spectrum of glyoxal in mammalian cells and to compare the spectrum with those observed in other experimental systems, we analyzed mutations in a bacterial suppressor tRNA gene (supF) in the shuttle vector plasmid pMY189. We treated pMY189 with glyoxal and immediately transfected it into simian COS-7 cells. The cytotoxicity and mutation frequency increased according to the dose of glyoxal. The majority of glyoxal-induced mutations (48%) were single-base substitutions. Eighty three percent of the single-base substitutions occurred at G:C base pairs. Among them, G:C-->T:A transversions were predominant, followed by G:C-->C:G transversions and G:C-->A:T transitions. A:T-->T:A transversions were also observed. Mutational hotspots within the supF gene were detected. These results suggest that glyoxal may play an important role in mutagenesis induced by oxygen free radicals.
|
| 巻・号 |
25(10)
|
| ページ |
1897-902
|
| 公開日 |
1997-5-15
|
| DOI |
10.1093/nar/25.10.1897
|
| PII |
gka331
|
| PMID |
9115355
|
| PMC |
PMC146688
|
| MeSH |
Animals
Base Composition
Base Sequence
COS Cells
Cell Survival / drug effects
Cytosine
DNA Replication
Genes, Bacterial
Genes, Suppressor / genetics*
Genetic Vectors*
Glyoxal / pharmacology*
Guanine
Mammals
Molecular Sequence Data
Mutagenesis
Mutagens / pharmacology*
Oligodeoxyribonucleotides
Plasmids / drug effects*
Point Mutation
RNA, Transfer / biosynthesis*
Sequence Deletion
Transfection
|
| IF |
11.502
|
| 引用数 |
61
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
COS-7(RCB0539) |
