論文 - 詳細
| RRC ID | 38982 |
|---|---|
| 著者 | Yamada T, Endo R, Gotoh M, Hirohashi S. |
| タイトル | Identification of semaphorin E as a non-MDR drug resistance gene of human cancers. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
To improve cancer chemotherapy, a better understanding of the molecular mechanisms of drug resistance is essential. To identify the molecules responsible for drug resistance that is unrelated to MDR1 or MRP gene products, a eukaryotic expression cDNA library of cis-diamminedichloroplatinum(II) (CDDP)-resistant ovarian cancer TYKnuR cells was introduced into Cos-7 cells. After repeated CDDP selection, cDNA homologous to murine semaphorin E was isolated from surviving cells. Human semaphorin E (H-sema E) was overexpressed in CDDP-resistant cell lines and was readily induced not only by diverse chemotherapeutic drugs but also by x-ray and UV irradiation. Transfection of H-sema E conferred a drug-resistant phenotype to CDDP-sensitive cells. In addition, the aberrant expression of H-sema E protein was detected immunohistochemically in 14 of 42 (33.3%) recurrent squamous cell carcinomas removed at autopsy after extensive radiochemotherapy. Recently, another member of the semaphorin family, CD100, was shown to significantly improve the viability of B lymphocytes. These results suggest the involvement of semaphorins in diverse cell survival mechanisms. |
| 巻・号 | 94(26) |
| ページ | 14713-8 |
| 公開日 | 1997-12-23 |
| DOI | 10.1073/pnas.94.26.14713 |
| PMID | 9405678 |
| PMC | PMC25101 |
| MeSH | Adult Amino Acid Sequence Animals Antineoplastic Agents / pharmacology Antineoplastic Agents / therapeutic use Carrier Proteins / genetics* Cisplatin / pharmacology Cisplatin / therapeutic use Cloning, Molecular DNA, Complementary / genetics Drug Resistance, Neoplasm / genetics* Female Gene Expression Regulation, Neoplastic Humans Mice Molecular Sequence Data Nerve Tissue Proteins / genetics* Ovarian Neoplasms / drug therapy Ovarian Neoplasms / genetics* Semaphorin-3A* Tumor Cells, Cultured |
| IF | 9.412 |
| 引用数 | 86 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 18 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | COS-7(RCB0539) |