RRC ID 38991
Author Kinto N, Iwamoto M, Enomoto-Iwamoto M, Noji S, Ohuchi H, Yoshioka H, Kataoka H, Wada Y, Yuhao G, Takahashi HE, Yoshiki S, Yamaguchi A.
Title Fibroblasts expressing Sonic hedgehog induce osteoblast differentiation and ectopic bone formation.
Journal FEBS Lett.
Abstract We investigated the role of Sonic hedgehog (SHH) in osteoblast differentiation and bone formation. The numbers of ALP-positive cells in the mouse fibroblastic cell line C3H10T1/2 and the mouse osteoblastic cell line MC3T3-E1 were increased by co-culture with chicken fibroblasts transfected with chicken Shh cDNA encoding amino-terminal peptide (Shh-N). The conditioned medium of Shh-N-RCAS-transfected chicken fibroblast cultures also significantly increased ALP activity in both C3H10T1/2 and MC3T3-E1 cells. Intramuscular transplantation of Shh-N-RCAS-transfected chicken fibroblasts into athymic mice induced ectopic bone formation. These results indicate that SHH induces osteoblast differentiation and ectopic bone formation.
Volume 404(2-3)
Pages 319-23
Published 1997-3-10
DOI 10.1016/s0014-5793(97)00014-8
PII S0014-5793(97)00014-8
PMID 9119087
MeSH 3T3 Cells Animals Bone Development* Cell Differentiation Cell Line Chick Embryo Coculture Techniques Embryonic Induction Fibroblasts / cytology Fibroblasts / metabolism Fibroblasts / transplantation Hedgehog Proteins Kinetics Mice Mice, Inbred C3H Mice, Nude Osteoblasts / cytology* Osteoblasts / transplantation Peptide Fragments / biosynthesis Protein Biosynthesis* Recombinant Proteins / biosynthesis Time Factors Trans-Activators* Transfection
IF 2.999
Times Cited 101
Human and Animal Cells