論文 - 詳細
| RRC ID | 39000 |
|---|---|
| 著者 | Nakatsu S, Kondo S, Kondo Y, Yin D, Peterson JW, Kaakaji R, Morimura T, Kikuchi H, Takeuchi J, Barnett GH. |
| タイトル | Induction of apoptosis in multi-drug resistant (MDR) human glioblastoma cells by SN-38, a metabolite of the camptothecin derivative CPT-11. |
| ジャーナル | Cancer Chemother Pharmacol |
| Abstract |
The overexpression of the multidrug resistance (mdr1) gene and its product, P-glycoprotein (P-gp), is thought to limit the successful chemotherapy of human tumors. Recent studies demonstrate that SN-38, a metabolite of the camptothecin (CPT) derivative CPT-11, has antitumor effects on several tumors, but the mechanisms responsible for its cytotoxicity remain unclear. We therefore determined whether SN-38 has cytotoxic effects on MDR human glioblastoma GB-1 cells and non-MDR human glioblastoma U87-MG cells. Furthermore, we determined what role SN-38 plays in the induction of cytotoxicity in these tumor cells. In this study, we demonstrated that SN-38 had significantly stronger antitumor effects on GB-1 and U-87MG cells than did CPT (P < 0.01 and P < 0.05, respectively). In addition, findings obtained using a DNA fragmentation assay, Hoechst 33258 staining, in situ end-labeling and cell cycle analysis demonstrated that SN-38 induced apoptosis in these tumors. Our results suggest that SN-38 has a stronger antitumor effect on malignant glioma cells regardless of MDR expression than does CPT, and therefore can be considered a new chemotherapeutic agent potentially effective in the treatment of human primary or recurrent malignant gliomas resistant to chemotherapy. |
| 巻・号 | 39(5) |
| ページ | 417-23 |
| 公開日 | 1997-1-1 |
| DOI | 10.1007/s002800050592 |
| PMID | 9054955 |
| MeSH | Antineoplastic Agents, Phytogenic / toxicity Apoptosis / drug effects* Camptothecin / analogs & derivatives* Camptothecin / toxicity Cell Cycle / drug effects Cell Line Cell Survival / drug effects* DNA, Neoplasm / drug effects Dose-Response Relationship, Drug Drug Resistance, Multiple* Flow Cytometry Glioblastoma Humans Irinotecan Kinetics Tumor Cells, Cultured |
| IF | 2.967 |
| 引用数 | 45 |
| WOS 分野 | PHARMACOLOGY & PHARMACY ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | U-87 MG(RCB0419) |