RRC ID 39009
著者 Komata T, Tanaka R, Yamamoto K, Oda T, Ono K, Yoshida S, Takahashi M.
タイトル B7-1(CD80)-transfected human glioma cells and interleukin-12 directly stimulate allogeneic CD8+ T cells.
ジャーナル J Immunother
Abstract To obtain more effective cytotoxic T lymphocytes (CTLs), we examined the effect of B7 costimulation and interleukin-12 (IL-12) on the induction of allogeneic CTLs. Peripheral blood lymphocytes (PBLs) or positively selected human CD8+ T cells (purity > 99%) from healthy donors were used as effector cells, and B7-1-transfected or nontransfected U251 human glioma cells (U251-B7 or U251-Vec.) were used as stimulator cells. In mixed lymphocyte culture (MLC) with PBLs and U251 cells, nonspecific natural killer (NK) activity was raised by addition of IL-12, and the effect of B7 costimulation was not observed. However, in MLC with CD8+ T cells, efficient proliferation, generation of CTLs, and cytokine production were induced by MLC with U251-B7 in the presence of IL-12. Efficient generation of CTLs was not induced by either MLC with U251-B7 alone or the addition of IL-12 alone. Our results indicate that a combination of B7-1 costimulation and IL-12 is effective for inducing generation of CTLs, and that the CD8+ T cells can be differentiated into CTLs without the help of CD4+ T cells or antigen-presenting cells or both.
巻・号 20(4)
ページ 256-64
公開日 1997-7-1
DOI 10.1097/00002371-199707000-00002
PMID 9220315
MeSH B7-1 Antigen / physiology* CD8-Positive T-Lymphocytes / drug effects* CD8-Positive T-Lymphocytes / immunology Cytokines / biosynthesis Flow Cytometry Humans Interleukin-12 / pharmacology* Killer Cells, Natural / immunology Lymphocyte Activation / drug effects* Transfection Tumor Cells, Cultured
IF 4.11
引用数 19
WOS 分野 MEDICINE, RESEARCH & EXPERIMENTAL IMMUNOLOGY ONCOLOGY
リソース情報
ヒト・動物細胞 K562(RCB0027) U251(RCB0461)