Reference - Detail
| RRC ID | 39009 |
|---|---|
| Author | Komata T, Tanaka R, Yamamoto K, Oda T, Ono K, Yoshida S, Takahashi M. |
| Title | B7-1(CD80)-transfected human glioma cells and interleukin-12 directly stimulate allogeneic CD8+ T cells. |
| Journal | J Immunother |
| Abstract |
To obtain more effective cytotoxic T lymphocytes (CTLs), we examined the effect of B7 costimulation and interleukin-12 (IL-12) on the induction of allogeneic CTLs. Peripheral blood lymphocytes (PBLs) or positively selected human CD8+ T cells (purity > 99%) from healthy donors were used as effector cells, and B7-1-transfected or nontransfected U251 human glioma cells (U251-B7 or U251-Vec.) were used as stimulator cells. In mixed lymphocyte culture (MLC) with PBLs and U251 cells, nonspecific natural killer (NK) activity was raised by addition of IL-12, and the effect of B7 costimulation was not observed. However, in MLC with CD8+ T cells, efficient proliferation, generation of CTLs, and cytokine production were induced by MLC with U251-B7 in the presence of IL-12. Efficient generation of CTLs was not induced by either MLC with U251-B7 alone or the addition of IL-12 alone. Our results indicate that a combination of B7-1 costimulation and IL-12 is effective for inducing generation of CTLs, and that the CD8+ T cells can be differentiated into CTLs without the help of CD4+ T cells or antigen-presenting cells or both. |
| Volume | 20(4) |
| Pages | 256-64 |
| Published | 1997-7-1 |
| DOI | 10.1097/00002371-199707000-00002 |
| PMID | 9220315 |
| MeSH | B7-1 Antigen / physiology* CD8-Positive T-Lymphocytes / drug effects* CD8-Positive T-Lymphocytes / immunology Cytokines / biosynthesis Flow Cytometry Humans Interleukin-12 / pharmacology* Killer Cells, Natural / immunology Lymphocyte Activation / drug effects* Transfection Tumor Cells, Cultured |
| IF | 4.11 |
| Times Cited | 19 |
| WOS Category | MEDICINE, RESEARCH & EXPERIMENTAL IMMUNOLOGY ONCOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | K562(RCB0027) U251(RCB0461) |