| RRC ID |
39009
|
| Author |
Komata T, Tanaka R, Yamamoto K, Oda T, Ono K, Yoshida S, Takahashi M.
|
| Title |
B7-1(CD80)-transfected human glioma cells and interleukin-12 directly stimulate allogeneic CD8+ T cells.
|
| Journal |
J Immunother
|
| Abstract |
To obtain more effective cytotoxic T lymphocytes (CTLs), we examined the effect of B7 costimulation and interleukin-12 (IL-12) on the induction of allogeneic CTLs. Peripheral blood lymphocytes (PBLs) or positively selected human CD8+ T cells (purity > 99%) from healthy donors were used as effector cells, and B7-1-transfected or nontransfected U251 human glioma cells (U251-B7 or U251-Vec.) were used as stimulator cells. In mixed lymphocyte culture (MLC) with PBLs and U251 cells, nonspecific natural killer (NK) activity was raised by addition of IL-12, and the effect of B7 costimulation was not observed. However, in MLC with CD8+ T cells, efficient proliferation, generation of CTLs, and cytokine production were induced by MLC with U251-B7 in the presence of IL-12. Efficient generation of CTLs was not induced by either MLC with U251-B7 alone or the addition of IL-12 alone. Our results indicate that a combination of B7-1 costimulation and IL-12 is effective for inducing generation of CTLs, and that the CD8+ T cells can be differentiated into CTLs without the help of CD4+ T cells or antigen-presenting cells or both.
|
| Volume |
20(4)
|
| Pages |
256-64
|
| Published |
1997-7-1
|
| DOI |
10.1097/00002371-199707000-00002
|
| PMID |
9220315
|
| MeSH |
B7-1 Antigen / physiology*
CD8-Positive T-Lymphocytes / drug effects*
CD8-Positive T-Lymphocytes / immunology
Cytokines / biosynthesis
Flow Cytometry
Humans
Interleukin-12 / pharmacology*
Killer Cells, Natural / immunology
Lymphocyte Activation / drug effects*
Transfection
Tumor Cells, Cultured
|
| IF |
4.11
|
| Times Cited |
19
|
|
WOS Category
|
MEDICINE, RESEARCH & EXPERIMENTAL
IMMUNOLOGY
ONCOLOGY
|
| Resource |
| Human and Animal Cells |
K562(RCB0027)
U251(RCB0461) |
