論文 - 詳細
| RRC ID | 39009 |
|---|---|
| 著者 | Komata T, Tanaka R, Yamamoto K, Oda T, Ono K, Yoshida S, Takahashi M. |
| タイトル | B7-1(CD80)-transfected human glioma cells and interleukin-12 directly stimulate allogeneic CD8+ T cells. |
| ジャーナル | J Immunother |
| Abstract |
To obtain more effective cytotoxic T lymphocytes (CTLs), we examined the effect of B7 costimulation and interleukin-12 (IL-12) on the induction of allogeneic CTLs. Peripheral blood lymphocytes (PBLs) or positively selected human CD8+ T cells (purity > 99%) from healthy donors were used as effector cells, and B7-1-transfected or nontransfected U251 human glioma cells (U251-B7 or U251-Vec.) were used as stimulator cells. In mixed lymphocyte culture (MLC) with PBLs and U251 cells, nonspecific natural killer (NK) activity was raised by addition of IL-12, and the effect of B7 costimulation was not observed. However, in MLC with CD8+ T cells, efficient proliferation, generation of CTLs, and cytokine production were induced by MLC with U251-B7 in the presence of IL-12. Efficient generation of CTLs was not induced by either MLC with U251-B7 alone or the addition of IL-12 alone. Our results indicate that a combination of B7-1 costimulation and IL-12 is effective for inducing generation of CTLs, and that the CD8+ T cells can be differentiated into CTLs without the help of CD4+ T cells or antigen-presenting cells or both. |
| 巻・号 | 20(4) |
| ページ | 256-64 |
| 公開日 | 1997-7-1 |
| DOI | 10.1097/00002371-199707000-00002 |
| PMID | 9220315 |
| MeSH | B7-1 Antigen / physiology* CD8-Positive T-Lymphocytes / drug effects* CD8-Positive T-Lymphocytes / immunology Cytokines / biosynthesis Flow Cytometry Humans Interleukin-12 / pharmacology* Killer Cells, Natural / immunology Lymphocyte Activation / drug effects* Transfection Tumor Cells, Cultured |
| IF | 4.11 |
| 引用数 | 19 |
| WOS 分野 | MEDICINE, RESEARCH & EXPERIMENTAL IMMUNOLOGY ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | K562(RCB0027) U251(RCB0461) |