| RRC ID |
39042
|
| Author |
Takahashi K, Imai A, Iijima M, Yoshimoto N, Maturana AD, Kuroda S, Niimi T.
|
| Title |
Mapping the heparin-binding site of the osteoinductive protein NELL1 by site-directed mutagenesis.
|
| Journal |
FEBS Lett
|
| Abstract |
Neural epidermal growth factor-like (NEL)-like 1 (NELL1) is a secretory osteogenic protein comprising an N-terminal thrombospondin-1-like (TSPN) domain, four von Willebrand factor type C domains, and six epidermal growth factor-like repeats. NELL1 shows heparin-binding activity; however, the biological significance remains to be explored. In this report, we demonstrate that NELL1 binds to cell surface proteoglycans through its TSPN domain. Major heparin-binding sites were identified on the three-dimensional structural model of the TSPN domain of NELL1. Mutant analysis of the heparin-binding sites indicated that the heparin-binding activity of the TSPN domain is involved in interaction of NELL1 with cell surface proteoglycans.
|
| Volume |
589(24 Pt B)
|
| Pages |
4026-32
|
| Published |
2015-12-21
|
| DOI |
10.1016/j.febslet.2015.11.032
|
| PII |
S0014-5793(15)01045-5
|
| PMID |
26627376
|
| MeSH |
Animals
Anticoagulants / metabolism*
Binding Sites
Calcium-Binding Proteins
Cell Line
Cell Membrane / metabolism*
Heparan Sulfate Proteoglycans / metabolism*
Heparin / metabolism*
Humans
Kinetics
Mesenchymal Stem Cells / metabolism
Mice
Models, Molecular*
Mutagenesis, Site-Directed
Mutant Proteins / chemistry
Mutant Proteins / metabolism
Nerve Tissue Proteins / chemistry
Nerve Tissue Proteins / genetics
Nerve Tissue Proteins / metabolism*
Osteoblasts / metabolism*
Protein Interaction Mapping
Recombinant Fusion Proteins / chemistry
Recombinant Fusion Proteins / metabolism
Species Specificity
Thrombospondin 1 / metabolism
|
| IF |
3.057
|
| Times Cited |
7
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| Human and Animal Cells |
10T1/2(RCB0247) |
