Reference - Detail
| RRC ID | 39056 |
|---|---|
| Author | Monzen S, Chiba M, Hosokawa Y. |
| Title | Genetic network profiles associated with established resistance to ionizing radiation in acute promyelocytic leukemia cells and their extracellular vesicles. |
| Journal | Oncol Rep |
| Abstract |
Radiation-resistant acute promyelocytic leukemia (APL) cells present challenges to treatment, and the acquisition of resistance to ionizing radiation (IR) is a matter of clinical concern. However, little information is available on the behavior of radio-resistant APL in terms of gene expression profiles and intercellular communication. In this study, cDNA microarray and RT-PCR were used to analyze the intracellular genetic network and extracellular vesicles (EVs), respectively, in the established radio-resistant HL60 (Res-HL60) cell line. Significant changes in the expression of 7,309 known mRNAs were observed in Res-HL60 relative to control. In addition, 7 mRNAs were determined as targets because significant changes in the expression were observed using Ingenuity analysis software, confirming the quantitative RT-PCR. However, EVs from Res-HL60 cells did not include these target molecules. These results suggest that radio-resistant APL is regulated by the expression and suppression of specific molecules, and these molecules are not transferred between cells by EVs. |
| Volume | 35(2) |
| Pages | 749-56 |
| Published | 2016-2-1 |
| DOI | 10.3892/or.2015.4471 |
| PMID | 26718911 |
| MeSH | Extracellular Vesicles / genetics* Gene Regulatory Networks / genetics* HL-60 Cells Humans Leukemia, Promyelocytic, Acute / genetics* Oligonucleotide Array Sequence Analysis Radiation Tolerance / genetics* Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Transcriptome |
| IF | 3.417 |
| Times Cited | 0 |
| WOS Category | ONCOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | HL60(RCB0041) |