RRC ID |
39063
|
著者 |
Nakashima S, Sugita Y, Miyoshi H, Arakawa F, Muta H, Ishibashi Y, Niino D, Ohshima K, Terasaki M, Nakamura Y, Morioka M.
|
タイトル |
Endothelin B receptor expression in malignant gliomas: the perivascular immune escape mechanism of gliomas.
|
ジャーナル |
J Neurooncol
|
Abstract |
In order to clarify the role of endothelin B receptors (ETBRs) in gliomas, we analyzed cell cultures and surgical specimens of gliomas using RT-PCR and immunohistochemistry. RT-PCR measured the absolute expression of ETBR mRNA in twelve samples, which included gliomas that were classified using the World Health Organization (WHO) classification system Grade I-IV, as well as two glioblastoma cell lines (CCF-STTG1 and U87-MG). Using immunohistochemistry, 77 glioma specimens were evaluated for their expression of ETBR and infiltrating T lymphocytes, including an analysis of cytotoxic T cells (CTLs) and regulatory T lymphocytes (Tregs). The number of ETBR-positive vessels in the glioblastomas (Grade IV) was significantly higher than in other grades of gliomas (comparisons to Grade IV, Grade I: p = 0.0323, Grade II: p = 0.0009, Grade III: p = 0.0273). The ETBR expression rate (defined as the number of ETBR-positive blood vessels divided by the total number of blood vessels) in the glioblastomas was higher than the ETBR expression rate in the low-grade gliomas (compared to Grade IV, Grade I: p = 0.0132, Grade II: p = 0.0018, Grade III: p = 0.0745). In addition, the cases which had an ETBR expression rate of 50 % or higher exhibited fewer infiltrating CTLs and more infiltrating Tregs compared to the cases with an ETBR expression rate <50 % (CTLs: p = 0.0342; Tregs: p = 0.0175). Isocitrate dehydrogenase 1 (IDH-1) mutations were identified in 21 cases, but there was no correlation between ETBR expression and IDH-1 mutations for any WHO grade. These results suggest that ETBR expression during neo-angiogenesis may interfere with the homing of CTLs around the tumor and be involved in the immune escape mechanism of gliomas.
|
巻・号 |
127(1)
|
ページ |
23-32
|
公開日 |
2016-3-1
|
DOI |
10.1007/s11060-015-2017-5
|
PII |
10.1007/s11060-015-2017-5
|
PMID |
26645886
|
MeSH |
Biomarkers, Tumor / genetics
Biomarkers, Tumor / metabolism*
Blotting, Western
Brain Neoplasms / genetics
Brain Neoplasms / immunology
Brain Neoplasms / metabolism
Brain Neoplasms / pathology*
Gene Expression Regulation, Neoplastic
Glioma / genetics
Glioma / immunology
Glioma / metabolism
Glioma / pathology*
Humans
Immunoenzyme Techniques
Isocitrate Dehydrogenase / genetics
Mutation / genetics
Neoplasm Grading
Neovascularization, Pathologic*
RNA, Messenger / genetics
Real-Time Polymerase Chain Reaction
Receptor, Endothelin B / genetics
Receptor, Endothelin B / metabolism*
Reverse Transcriptase Polymerase Chain Reaction
T-Lymphocytes, Cytotoxic / immunology*
Tumor Cells, Cultured
|
IF |
3.267
|
引用数 |
4
|
WOS 分野
|
CLINICAL NEUROLOGY
ONCOLOGY
|
リソース情報 |
ヒト・動物細胞 |
CCF-STTG1(RCB1977) |