| RRC ID |
39080
|
| 著者 |
Hoq MM, Suzutani T, Toyoda T, Horiike G, Yoshida I, Azuma M.
|
| タイトル |
Role of gamma delta TCR+ lymphocytes in the augmented resistance of trehalose 6,6'-dimycolate-treated mice to influenza virus infection.
|
| ジャーナル |
J Gen Virol
|
| Abstract |
Trehalose 6,6'-dimycolate (TDM), an immunomodulator, potentiates non-specific resistance in mice to influenza virus infection. When mice were injected intravenously with TDM, the striking proliferation of a minority of T-lymphocytes bearing gamma/delta T-cell receptors (gamma delta T-cells) that accumulated in granulomatous lungs was thought to be associated with the maintenance of acquired resistance to lethal influenza virus infection. To clarify the cellular basis of the defence against influenza virus, mice were depleted of gamma delta T-cells, alpha/beta (alpha beta) T-cells, or natural killer (NK) cells by in vivo administration of corresponding antibodies prior to influenza virus infection. The depletion of gamma delta T-cells significantly abrogated the augmented resistance of TDM-treated mice to infection, as did depletion of either alpha beta T-cells or NK cells. To gain insight into the functional ability of gamma delta T-cells, we evaluated the cytotoxic activity of this T-cell subset against a panel of target cell lines that were stably transfected with the influenza virus haemagglutinin (HA) gene from A/PR/8/34(H1N1) and A/Aichi/2/68(H3N2) strains. The gamma delta T-cells from TDM-treated mice showed profound cytotoxicity against the target cells expressing HA of either the H1 or H3 subtype, in a non-major histocompatibility complex-restricted manner. Taken together, these results indicate that gamma delta T-cells play a non-specific role, in conjunction with alpha beta T-cells and NK cells, in protecting mice against influenza virus infection, and that the recognition and destruction of HA-expressing target cells by the activated gamma delta T-cells is one of the steps involved in this anti-influenza virus immunosurveillance.
|
| 巻・号 |
78 ( Pt 7)
|
| ページ |
1597-603
|
| 公開日 |
1997-7-1
|
| DOI |
10.1099/0022-1317-78-7-1597
|
| PMID |
9225035
|
| MeSH |
Adjuvants, Immunologic / administration & dosage*
Animals
Cells, Cultured
Chick Embryo
Cord Factors / administration & dosage*
Cytotoxicity Tests, Immunologic
Disease Models, Animal
Female
Hemagglutinin Glycoproteins, Influenza Virus / genetics
Hemagglutinin Glycoproteins, Influenza Virus / immunology
Humans
Immunity, Innate
Influenza A virus / immunology
Influenza, Human / immunology*
Killer Cells, Natural / immunology
Lymphocyte Depletion
Mice
Mice, Inbred BALB C
Receptors, Antigen, T-Cell, alpha-beta / immunology
Receptors, Antigen, T-Cell, gamma-delta / immunology*
T-Lymphocytes / cytology
T-Lymphocytes / immunology*
|
| IF |
3.376
|
| 引用数 |
27
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
VIROLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
B16 melanoma 4A5(RCB0557) |
