| RRC ID |
39086
|
| 著者 |
Hirano F, Hirano H, Hino E, Takayama S, Saito K, Kusumoto Y, Shimabukuro Y, Murakami S, Okada H.
|
| タイトル |
CD44 isoform expression in periodontal tissues: cell-type specific regulation of alternative splicing.
|
| ジャーナル |
J Periodontal Res
|
| Abstract |
CD44 functions as a receptor for various extracellular matrices and plays crucial roles in homotypic and heterotypic cell-cell interactions. Recently, the molecular structure of CD44 has been extensively analyzed and multiple isoforms produced by alternative splicing of messenger RNA have been identified. In this study, we examined the expression of CD44 isoforms on different cell types isolated from periodontal tissue. In order to examine tissue differences in CD44 isoform expression, we established in vitro cell culture of human gingival fibroblasts (HGF), human periodontal ligament cells (HPDL) and human gingival epithelial cells (HGEC). These cells all expressed CD44 protein and messenger RNA. However, immunoprecipitation and Northern blot analysis revealed that HGEC expressed larger CD44 isoforms than HGF and HPDL. Reverse transcription-polymerase chain reaction with primers flanking the insertion site of alternatively spliced exons was used to study details of the heterogeneity. All cells examined expressed a major band in the absence of alternatively spliced exons and additional larger bands. In particular, HGEC contained more abundant high molecular mass species. In vitro stimulation by IL-1 beta, TNF alpha or phorbol 12-myristate 13-acetate induced an increase in total CD44 messenger RNA in HGF but not change in overall patterns of CD44 isoform expression. However, the isoform expression of HGEC was sensitive to cell density. The amount of larger isoform was decreased by culturing cells beyond confluence. These findings suggest that CD44 isoform expression is cell type-specifically regulated in periodontium and altered according to growth phase of HGEC.
|
| 巻・号 |
32(8)
|
| ページ |
634-45
|
| 公開日 |
1997-11-1
|
| DOI |
10.1111/j.1600-0765.1997.tb00573.x
|
| PMID |
9409458
|
| MeSH |
Alternative Splicing*
Blotting, Northern
Cell Communication
Cell Count
Cell Cycle / genetics
Cells, Cultured
DNA Primers
Epithelial Cells / cytology
Epithelial Cells / immunology
Exons / genetics
Extracellular Matrix / immunology
Fibroblasts / cytology
Fibroblasts / immunology
Gene Expression Regulation
Gingiva / cytology
Gingiva / immunology*
Humans
Hyaluronan Receptors / analysis
Hyaluronan Receptors / classification
Hyaluronan Receptors / genetics*
Interleukin-1 / pharmacology
Periodontal Ligament / cytology
Periodontal Ligament / immunology*
Polymerase Chain Reaction
Precipitin Tests
RNA, Messenger / genetics
Tetradecanoylphorbol Acetate / pharmacology
Transcription, Genetic
Tumor Necrosis Factor-alpha / pharmacology
|
| IF |
2.926
|
| 引用数 |
13
|
|
WOS 分野
|
DENTISTRY, ORAL SURGERY & MEDICINE
|
| リソース情報 |
| ヒト・動物細胞 |
A549(RCB0098) |
