RRC ID 39086
Author Hirano F, Hirano H, Hino E, Takayama S, Saito K, Kusumoto Y, Shimabukuro Y, Murakami S, Okada H.
Title CD44 isoform expression in periodontal tissues: cell-type specific regulation of alternative splicing.
Journal J Periodontal Res
Abstract CD44 functions as a receptor for various extracellular matrices and plays crucial roles in homotypic and heterotypic cell-cell interactions. Recently, the molecular structure of CD44 has been extensively analyzed and multiple isoforms produced by alternative splicing of messenger RNA have been identified. In this study, we examined the expression of CD44 isoforms on different cell types isolated from periodontal tissue. In order to examine tissue differences in CD44 isoform expression, we established in vitro cell culture of human gingival fibroblasts (HGF), human periodontal ligament cells (HPDL) and human gingival epithelial cells (HGEC). These cells all expressed CD44 protein and messenger RNA. However, immunoprecipitation and Northern blot analysis revealed that HGEC expressed larger CD44 isoforms than HGF and HPDL. Reverse transcription-polymerase chain reaction with primers flanking the insertion site of alternatively spliced exons was used to study details of the heterogeneity. All cells examined expressed a major band in the absence of alternatively spliced exons and additional larger bands. In particular, HGEC contained more abundant high molecular mass species. In vitro stimulation by IL-1 beta, TNF alpha or phorbol 12-myristate 13-acetate induced an increase in total CD44 messenger RNA in HGF but not change in overall patterns of CD44 isoform expression. However, the isoform expression of HGEC was sensitive to cell density. The amount of larger isoform was decreased by culturing cells beyond confluence. These findings suggest that CD44 isoform expression is cell type-specifically regulated in periodontium and altered according to growth phase of HGEC.
Volume 32(8)
Pages 634-45
Published 1997-11-1
DOI 10.1111/j.1600-0765.1997.tb00573.x
PMID 9409458
MeSH Alternative Splicing* Blotting, Northern Cell Communication Cell Count Cell Cycle / genetics Cells, Cultured DNA Primers Epithelial Cells / cytology Epithelial Cells / immunology Exons / genetics Extracellular Matrix / immunology Fibroblasts / cytology Fibroblasts / immunology Gene Expression Regulation Gingiva / cytology Gingiva / immunology* Humans Hyaluronan Receptors / analysis Hyaluronan Receptors / classification Hyaluronan Receptors / genetics* Interleukin-1 / pharmacology Periodontal Ligament / cytology Periodontal Ligament / immunology* Polymerase Chain Reaction Precipitin Tests RNA, Messenger / genetics Tetradecanoylphorbol Acetate / pharmacology Transcription, Genetic Tumor Necrosis Factor-alpha / pharmacology
IF 2.926
Times Cited 13
Human and Animal Cells A549(RCB0098)