Reference - Detail
| RRC ID | 39107 |
|---|---|
| Author | Kanda T, Foucand L, Nakamura Y, Niot I, Besnard P, Fujita M, Sakai Y, Hatakeyama K, Ono T, Fujii H. |
| Title | Regulation of expression of human intestinal bile acid-binding protein in Caco-2 cells. |
| Journal | Biochem J |
| Abstract |
Molecular mechanisms of the bile acid active transport system in the ileal enterocytes remain unknown. We examined whether bile acids affect human enterocyte gene expression of intestinal bile acid-binding protein (I-BABP), a component of this transport system. Differentiated Caco-2 cells were incubated in the presence of human bile, bile acids or other lipids. The level of I-BABP expression was evaluated by Northern and Western blot analyses. A 24 h incubation of Caco-2 cells in a medium containing either bile or bile acids resulted in a remarkable 7.5-fold increase in the I-BABP mRNA level over the control level. Neither cholesterol, palmitic acid, phosphatidylcholine nor cholestyramine treated bile showed any difference in I-BABP mRNA expression from the control. Bile acid treatment increased the level of I-BABP mRNA in Caco-2 cells in a time- and dose-dependent manner. Western blot analysis showed that this induction led to increase in cytosolic I-BABP. Chenodeoxycholic acid and deoxycholic acid showed greater induction effects than other hydrophilic bile acids, including their own glycine conjugates. Pretreatment by actinomycin D or cycloheximide completely inhibited the up-regulation of I-BABP expression by bile acid. Bile acids, especially lipophilic bile acids, increase the I-BABP expression in Caco-2-cells, suggesting that luminal bile acids play an important role in regulating the I-BABP gene expression. |
| Volume | 330 ( Pt 1)(Pt 1) |
| Pages | 261-5 |
| Published | 1998-2-15 |
| DOI | 10.1042/bj3300261 |
| PMID | 9461519 |
| PMC | PMC1219136 |
| MeSH | 3-alpha-Hydroxysteroid Dehydrogenase (B-Specific) Bile Acids and Salts / physiology* Caco-2 Cells / metabolism* Carrier Proteins / genetics Carrier Proteins / metabolism* Cytosol / metabolism Dose-Response Relationship, Drug Fatty Acid-Binding Protein 7 Fatty Acid-Binding Proteins Female Gene Expression Regulation, Neoplastic Humans Hydroxysteroid Dehydrogenases* Ileum / physiology* Membrane Glycoproteins* Middle Aged Myelin P2 Protein / genetics Neoplasm Proteins* Protein Synthesis Inhibitors / pharmacology RNA, Messenger / genetics RNA, Neoplasm / genetics Transcription, Genetic / drug effects Tumor Suppressor Proteins* Up-Regulation |
| IF | 4.097 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 13 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | CACO-2(ECA86010202) |