論文 - 詳細
| RRC ID | 39112 |
|---|---|
| 著者 | Goto H, Kosako H, Tanabe K, Yanagida M, Sakurai M, Amano M, Kaibuchi K, Inagaki M. |
| タイトル | Phosphorylation of vimentin by Rho-associated kinase at a unique amino-terminal site that is specifically phosphorylated during cytokinesis. |
| ジャーナル | J Biol Chem |
| Abstract |
We found that vimentin, the most widely expressed intermediate filament protein, served as an excellent substrate for Rho-associated kinase (Rho-kinase) and that vimentin phosphorylated by Rho-kinase lost its ability to form filaments in vitro. Two amino-terminal sites on vimentin, Ser38 and Ser71, were identified as the major phosphorylation sites for Rho-kinase, and Ser71 was the most favored and unique phosphorylation site for Rho-kinase in vitro. To analyze the vimentin phosphorylation by Rho-kinase in vivo, we prepared an antibody GK71 that specifically recognizes the phosphorylation of vimentin-Ser71. Ectopic expression of constitutively active Rho-kinase in COS-7 cells induced phosphorylation of vimentin at Ser71, followed by the reorganization of vimentin filament networks. During the cell cycle, the phosphorylation of vimentin-Ser71 occurred only at the cleavage furrow in late mitotic cells but not in interphase or early mitotic cells. This cleavage furrow-specific phosphorylation of vimentin-Ser71 was observed in the various types of cells we examined. All these accumulating observations increase the possibility that Rho-kinase may have a definite role in governing regulatory processes in assembly-disassembly and turnover of vimentin filaments at the cleavage furrow during cytokinesis. |
| 巻・号 | 273(19) |
| ページ | 11728-36 |
| 公開日 | 1998-5-8 |
| DOI | 10.1074/jbc.273.19.11728 |
| PII | S0021-9258(19)89381-X |
| PMID | 9565595 |
| MeSH | Amino Acid Sequence Anaphase Animals COS Cells Cattle Cell Division* Fluorescent Antibody Technique, Indirect GTP-Binding Proteins / metabolism Guanosine Triphosphate / metabolism Immunologic Techniques Intermediate Filaments / ultrastructure Intracellular Signaling Peptides and Proteins Microscopy, Confocal Molecular Sequence Data Phosphorylation Phosphoserine / metabolism Protein Serine-Threonine Kinases / metabolism* Vimentin / metabolism* rho GTP-Binding Proteins rho-Associated Kinases |
| IF | 4.238 |
| 引用数 | 169 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | COS-7(RCB0539) |