| RRC ID |
39112
|
| Author |
Goto H, Kosako H, Tanabe K, Yanagida M, Sakurai M, Amano M, Kaibuchi K, Inagaki M.
|
| Title |
Phosphorylation of vimentin by Rho-associated kinase at a unique amino-terminal site that is specifically phosphorylated during cytokinesis.
|
| Journal |
J Biol Chem
|
| Abstract |
We found that vimentin, the most widely expressed intermediate filament protein, served as an excellent substrate for Rho-associated kinase (Rho-kinase) and that vimentin phosphorylated by Rho-kinase lost its ability to form filaments in vitro. Two amino-terminal sites on vimentin, Ser38 and Ser71, were identified as the major phosphorylation sites for Rho-kinase, and Ser71 was the most favored and unique phosphorylation site for Rho-kinase in vitro. To analyze the vimentin phosphorylation by Rho-kinase in vivo, we prepared an antibody GK71 that specifically recognizes the phosphorylation of vimentin-Ser71. Ectopic expression of constitutively active Rho-kinase in COS-7 cells induced phosphorylation of vimentin at Ser71, followed by the reorganization of vimentin filament networks. During the cell cycle, the phosphorylation of vimentin-Ser71 occurred only at the cleavage furrow in late mitotic cells but not in interphase or early mitotic cells. This cleavage furrow-specific phosphorylation of vimentin-Ser71 was observed in the various types of cells we examined. All these accumulating observations increase the possibility that Rho-kinase may have a definite role in governing regulatory processes in assembly-disassembly and turnover of vimentin filaments at the cleavage furrow during cytokinesis.
|
| Volume |
273(19)
|
| Pages |
11728-36
|
| Published |
1998-5-8
|
| DOI |
10.1074/jbc.273.19.11728
|
| PII |
S0021-9258(19)89381-X
|
| PMID |
9565595
|
| MeSH |
Amino Acid Sequence
Anaphase
Animals
COS Cells
Cattle
Cell Division*
Fluorescent Antibody Technique, Indirect
GTP-Binding Proteins / metabolism
Guanosine Triphosphate / metabolism
Immunologic Techniques
Intermediate Filaments / ultrastructure
Intracellular Signaling Peptides and Proteins
Microscopy, Confocal
Molecular Sequence Data
Phosphorylation
Phosphoserine / metabolism
Protein Serine-Threonine Kinases / metabolism*
Vimentin / metabolism*
rho GTP-Binding Proteins
rho-Associated Kinases
|
| IF |
4.238
|
| Times Cited |
169
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
COS-7(RCB0539) |
