| RRC ID |
39118
|
| Author |
Ikeda M, Tsuji N, Kikukawa K, Asahara Y, Nakashima A, Minatogawa Y.
|
| Title |
DNA-binding properties of the overexpressed recombinant estrogen receptor alpha.
|
| Journal |
Biochem Mol Biol Int
|
| Abstract |
Estrogen receptor alpha was overexpressed in COS-7 cells by transformation of an expression vector with the full length open reading frame of the receptor alpha. The nuclear estradiol-receptor complex and the soluble receptor from the COS-7 cells were cross-linked with an estrogen response element (ERE), which was substituted with 5-bromo-2'-deoxyuridine (BrdUVRE), as the receptor dimers by UV irradiation. In gel retardation analysis, the specific bindings of both nuclear and soluble receptors to ERE were decreased with increasing of KCl concentration compared with 0.1 M KCl. The ionic interactions of both receptors with ERE are thought to be similar.
|
| Volume |
45(4)
|
| Pages |
673-80
|
| Published |
1998-7-1
|
| DOI |
10.1080/15216549800203072
|
| PMID |
9713689
|
| MeSH |
Animals
Antibodies, Monoclonal
Bromodeoxyuridine
COS Cells
DNA / genetics
DNA / metabolism*
Dimerization
Estradiol / metabolism
Estrogen Receptor alpha
Gene Expression
Potassium Chloride / pharmacology
Receptors, Estrogen / chemistry
Receptors, Estrogen / genetics
Receptors, Estrogen / immunology
Receptors, Estrogen / metabolism*
Recombinant Proteins / metabolism
Regulatory Sequences, Nucleic Acid*
Solubility
Ultraviolet Rays
|
| Times Cited |
2
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
|
