| RRC ID |
39131
|
| Author |
Yasuda H, Shima N, Nakagawa N, Mochizuki SI, Yano K, Fujise N, Sato Y, Goto M, Yamaguchi K, Kuriyama M, Kanno T, Murakami A, Tsuda E, Morinaga T, Higashio K.
|
| Title |
Identity of osteoclastogenesis inhibitory factor (OCIF) and osteoprotegerin (OPG): a mechanism by which OPG/OCIF inhibits osteoclastogenesis in vitro.
|
| Journal |
Endocrinology
|
| Abstract |
The morphogenesis and remodeling of bone depends on the integrated activity of osteoblasts that form bone and osteoclasts that resorb bone. We previously reported the isolation of a new cytokine termed osteoclastogenesis inhibitory factor, OCIF, which specifically inhibits osteoclast development. Here we report the cloning of a complementary DNA of human OCIF. OCIF is identical to osteoprotegerin (OPG), a soluble member of the tumor-necrosis factor receptor family that inhibits osteoclastogenesis. Recombinant human OPG/OCIF specifically acts on bone tissues and increases bone mineral density and bone volume associated with a decrease of active osteoclast number in normal rats. Osteoblasts or bone marrow-derived stromal cells support osteoclastogenesis through cell-to-cell interactions. A single class of high affinity binding sites for OPG/OCIF appears on a mouse stromal cell line, ST2, in response to 1,25-dihydroxyvitamin D3. An anti-OPG/OCIF antibody that blocks the binding abolishes the biological activity of OPG/OCIF. When the sites are blocked with OPG/OCIF, ST2 cells fail to support osteoclastogenesis. These results suggest that the sites are involved in cell-to-cell signaling between stromal cells and osteoclast progenitors and that OPG/OCIF inhibits osteoclastogenesis by interrupting the signaling through the sites.
|
| Volume |
139(3)
|
| Pages |
1329-37
|
| Published |
1998-3-1
|
| DOI |
10.1210/endo.139.3.5837
|
| PMID |
9492069
|
| MeSH |
Amino Acid Sequence
Animals
Bone Density / drug effects
Bone Remodeling
Cells, Cultured
Cloning, Molecular
Glycoproteins / genetics
Glycoproteins / physiology*
Humans
Male
Mice
Molecular Sequence Data
Osteoclasts / drug effects
Osteoclasts / physiology*
Osteoprotegerin
Rats
Rats, Sprague-Dawley
Receptors, Cytoplasmic and Nuclear*
Receptors, Tumor Necrosis Factor / genetics
Receptors, Tumor Necrosis Factor / physiology*
Recombinant Proteins / pharmacology
|
| IF |
3.934
|
| Times Cited |
944
|
|
WOS Category
|
ENDOCRINOLOGY & METABOLISM
|
| Resource |
| Human and Animal Cells |
MC3T3-E1(RCB1126)
ST2(RCB0224) |
