| RRC ID |
39138
|
| 著者 |
Okaichi K, Wang LH, Ihara M, Okumura Y.
|
| タイトル |
Sensitivity to ionizing radiation in Saos-2 cells transfected with mutant p53 genes depends on the mutation position.
|
| ジャーナル |
J Radiat Res
|
| Abstract |
We have constructed an in vitro system to examine how p53 mutants affect radiosensitivity. Mutations of p53 were made using in vitro mutagenesis, and mutant cDNAs were introduced into the human osteosarcoma cell line, Saos-2, which is devoid of endogenous p53. For wild type p53, both the expression plasmid and a regulation plasmid (LacSwitch system) were transfected into the cells. The radiosensitivities of clones of mutant p53 and wild type p53 were examined. Transformants of wild type p53 had increased radiosensitivity. The induction of wild type p53 protein by addition of IPTG did not significantly increased radiosensitivity. A mutation at codon 123 also increased radiosensitivity. Mutations at codons 143, 175, and 273 did not alter radiosensitivity.
|
| 巻・号 |
39(2)
|
| ページ |
111-8
|
| 公開日 |
1998-6-1
|
| DOI |
10.1269/jrr.39.111
|
| PMID |
9735599
|
| MeSH |
Genes, p53 / physiology*
Humans
Mutation
Osteosarcoma / pathology
Osteosarcoma / radiotherapy
Radiation Tolerance*
Transfection
Tumor Cells, Cultured
|
| IF |
1.95
|
| 引用数 |
18
|
|
WOS 分野
|
RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
Saos-2(RCB0428) |
