RRC ID 39141
著者 Satoh J, Kurohara K, Yukitake M, Kuroda Y.
タイトル Interleukin-15, a T-cell growth factor, is expressed in human neural cell lines and tissues.
ジャーナル J Neurol Sci
Abstract Interleukin-15 (IL-15) is a novel cytokine which shares activities and receptor components with IL-2. To investigate the biological roles of IL-15 in the human nervous system, we examined the expression of mRNAs for IL-15 and the IL-15 receptor three subunits (IL-15alpha, IL-2Rbeta and IL-2Rgamma) in human neural cell lines and tissues using reverse transcription-polymerase chain reaction and Southern blot analysis. The constitutive expression of high levels of IL-15 mRNA was observed in all the cell lines examined, including Y79 retinoblastoma, IMR-32 neuroblastoma, SK-N-SH neuroblastoma, U-373MG glioma, KG-1-C glioma, NTera2 teratocarcinoma and neurons derived from NTera2 cells following treatment with retinoic acid (RA). Among these cell lines, IL-15 protein was detectable at high levels in culture supernatants of SK-N-SH cells and NTera2-derived neurons. The expression of an alternatively-spliced transcript of the IL-15 gene was up-regulated in NTera2 cells during RA-induced neuronal differentiation, suggesting the existence of differentiation-dependent transcriptional regulation. The expression of IL-15 mRNA was also identified in the human cerebral and cerebellar tissues, peripheral nerve and skeletal muscle, while the mRNAs for the complete set of IL-15R components were detectable only in U-373MG cells, cerebral and cerebellar tissues at significant levels. These results indicate that the expression of IL-15 but not of IL-15R mRNA is universal in human neural cell lines and tissues and raise the possibility that IL-15 acts as a neuroimmune regulatory factor in the human central nervous system.
巻・号 155(2)
ページ 170-7
公開日 1998-3-5
DOI 10.1016/s0022-510x(97)00310-9
PII S0022510X97003109
PMID 9562262
MeSH Blotting, Southern Cell Line Enzyme-Linked Immunosorbent Assay Humans Interleukin-15 / biosynthesis* Microglia / metabolism Neurons / metabolism* Polymerase Chain Reaction Proteins / metabolism RNA, Messenger / biosynthesis T-Lymphocytes / metabolism*
IF 3.115
引用数 41
WOS 分野 CLINICAL NEUROLOGY NEUROSCIENCES
リソース情報
ヒト・動物細胞 HeLa(RCB0007) A549(RCB0098) Hep G2(RCB0459) KG-1-C(RCB0270)