RRC ID 39159
著者 Hosain MZ, Mori T, Kishimura A, Katayama Y.
タイトル Synergy between phenotypic modulation and ROS neutralization in reduction of inflammatory response of hypoxic microglia by using phosphatidylserine and antioxidant containing liposomes.
ジャーナル J Biomater Sci Polym Ed
Abstract Neuroinflammation caused by microglial activation is a key contributing factor in neurological disorders such as those involving ischaemia. Excess production of reactive oxygen species (ROS) and nitric oxide (NO) stimulates the inflammatory response during ischaemia, significantly damaging cells. Inhibition of inflammatory activation of microglia is a promising potential treatment approach for neurological diseases. In this study, we introduce α-tocopherol and phosphatidylserine (PS) containing liposomes (PST-liposomes) to inhibit the microglial inflammatory response. PS is known to have anti-inflammatory effects on microglia by modulating the microglial phenotype, while α-tocopherol is an antioxidant, known to neutralize ROS. We found that both PS-containing liposomes (PS-liposomes) and PST-liposomes, as compared with phosphatidylcholine containing liposomes, significantly increased viability of hypoxia-treated microglia. The PST-liposomes functioned better than the PS-liposomes and we attribute this superior effect to a synergy between PS and α-tocopherol. This synergic action of PST-liposomes was illustrated in their ability, when incubated with microglia, to reduce NO and pro-inflammatory cytokine (TNF-α) production and increase anti-inflammatory cytokine (TGF-β1) production. Thus, the improved viability of hypoxia-treated microglia when treated with PST-liposomes involved anti-inflammatory effects, including ROS neutralization, as well as induction of a microglial phenotypic change. Our results suggest that PST-liposomes represent a potential therapeutic approach to reducing ischaemic injury in the brain.
巻・号 27(3)
ページ 290-302
公開日 2016-1-1
DOI 10.1080/09205063.2015.1125565
PMID 26689775
MeSH Animals Anti-Inflammatory Agents / administration & dosage Antioxidants / administration & dosage Brain Cattle Cell Hypoxia / drug effects Cell Hypoxia / physiology Cell Line Cell Survival / drug effects Chickens Egg Proteins / chemistry Escherichia coli Lipopolysaccharides / toxicity Liposomes Mice Microglia / drug effects Microglia / immunology* Nitric Oxide / metabolism* Phosphatidylserines / administration & dosage Reactive Oxygen Species / metabolism* Transforming Growth Factor beta1 / metabolism Tumor Necrosis Factor-alpha / metabolism alpha-Tocopherol / administration & dosage
IF 2.69
引用数 7
WOS 分野 ENGINEERING, BIOMEDICAL POLYMER SCIENCE MATERIALS SCIENCE, BIOMATERIALS
リソース情報
ヒト・動物細胞 MG6(RCB2403)