RRC ID 39160
著者 Horigome S, Yoshida I, Ito S, Inohana S, Fushimi K, Nagai T, Yamaguchi A, Fujita K, Satoyama T, Katsuda SI, Suzuki S, Watai M, Hirose N, Mitsue T, Shirakawa H, Komai M.
タイトル Inhibitory effects of Kaempferia parviflora extract on monocyte adhesion and cellular reactive oxygen species production in human umbilical vein endothelial cells.
ジャーナル Eur J Nutr
Abstract PURPOSE:The rhizome of Kaempferia parviflora (KP) is used in traditional Thai medicine. In this study, we investigated the effects of an ethanol KP extract and two of its components [5,7-dimethoxyflavone (DMF) and 5-hydroxy-3,7,3',4'-tetramethoxyflavone (TMF)] on monocyte adhesion and cellular reactive oxygen species (ROS) production in human umbilical vein endothelial cells (HUVECs), which provide an in vitro model of events relevant to the development and progression of atherosclerosis.
METHODS:RAW264.7 mouse macrophage-like cells were incubated with various concentrations of KP extract or polymethoxyflavonoids and stimulated with lipopolysaccharide prior to measuring nitrite levels in the culture media. Monocyte adhesion was evaluated by measuring the fluorescently labeled human monocytic leukemia THP-1 cells that is attached to tumor necrosis factor-α (TNF-α)-stimulated HUVECs. Cellular ROS production was assessed by measuring cellular antioxidant activity using pyocyanin-stimulated HUVECs.
RESULTS:KP extract and DMF reduced nitrite levels (as indicator of nitric oxide production) in LPS-stimulated RAW264.7 cells and also inhibited THP-1 cell adhesion to HUVECs. These treatments induced mRNA expression of endothelial nitric oxide synthase in TNF-α-stimulated HUVECs and downregulated that of various cell adhesion molecules, inflammatory mediators, and endothelial function-related genes. Angiotensin-converting enzyme activity was inhibited by KP extract in vitro. Furthermore, KP extract, DMF, and TMF inhibited the production of cellular ROS in pyocyanin-stimulated HUVECs.
CONCLUSION:KP extract, DMF, and TMF showed potential anti-inflammatory and antioxidant effects in these in vitro models, properties that would inhibit the development and progression of atherosclerosis.
巻・号 56(3)
ページ 949-964
公開日 2017-4-1
DOI 10.1007/s00394-015-1141-5
PII 10.1007/s00394-015-1141-5
PMID 26704713
MeSH Animals Anti-Inflammatory Agents / pharmacology Antioxidants / pharmacology Cell Adhesion / drug effects* Down-Regulation Flavonoids / pharmacology Human Umbilical Vein Endothelial Cells / drug effects* Humans Lipopolysaccharides / metabolism Mice Monocytes / cytology Monocytes / drug effects* Nitric Oxide / metabolism Nitric Oxide Synthase Type III / genetics Nitric Oxide Synthase Type III / metabolism Plant Extracts / pharmacology* RAW 264.7 Cells RNA, Messenger / genetics RNA, Messenger / metabolism Reactive Oxygen Species / metabolism* Tumor Necrosis Factor-alpha / genetics Tumor Necrosis Factor-alpha / metabolism Zingiberaceae / chemistry*
IF 4.664
引用数 14
WOS 分野 NUTRITION & DIETETICS
リソース情報
ヒト・動物細胞 RAW 264(RCB0535)