RRC ID 392
Author Cunliffe VT.
Title Histone deacetylase 1 is required to repress Notch target gene expression during zebrafish neurogenesis and to maintain the production of motoneurones in response to hedgehog signalling.
Journal Development
Abstract Histone deacetylases (Hdacs) are widely implicated as key components of transcriptional silencing mechanisms. Here, I show that hdac1 is specifically required in the zebrafish embryonic CNS to maintain neurogenesis. In hdac1 mutant embryos, the Notch-responsive E(spl)-related neurogenic gene her6 is ectopically expressed at distinct sites within the developing CNS and proneural gene expression is correspondingly reduced or eliminated. Using an hdac1-specific morpholino, I show that this requirement for hdac1 is epistatic to the requirement for Notch signalling. Consequently, hdac1-deficient embryos exhibit several defects of neuronal specification and patterning, including a dramatic deficit of hedgehog-dependent branchiomotor neurones that is refractory to elevated levels of hedgehog signalling. Thus, in the zebrafish embryo, hdac1 is an essential component of the transcriptional silencing machinery that supports the formation and subsequent differentiation of neuronal precursors.
Volume 131(12)
Pages 2983-95
Published 2004-6
DOI 10.1242/dev.01166
PII 131/12/2983
PMID 15169759
MeSH Animals Cell Division Cloning, Molecular Embryo, Nonmammalian / physiology Gene Deletion Gene Expression Regulation, Developmental / genetics* Hedgehog Proteins Histone Deacetylase 1 Histone Deacetylases / deficiency Histone Deacetylases / genetics* Histone Deacetylases / metabolism* In Situ Hybridization Membrane Proteins / genetics* Morphogenesis / genetics* Motor Neurons / cytology Motor Neurons / physiology* Nervous System / embryology* Oligonucleotides, Antisense / pharmacology RNA, Messenger / genetics Receptors, Notch Signal Transduction Trans-Activators / physiology* Zebrafish / embryology Zebrafish / genetics* Zebrafish Proteins / genetics* Zebrafish Proteins / metabolism*
IF 5.413
Times Cited 81
Zebrafish rw0