RRC ID 39202
著者 Kasai T, Ohguchi K, Nakashima S, Ito Y, Naganawa T, Kondo N, Nozawa Y.
タイトル Increased activity of oleate-dependent type phospholipase D during actinomycin D-induced apoptosis in Jurkat T cells.
ジャーナル J Immunol
Abstract Apoptosis is an active form of cell death that can be induced by a wide variety of agents and conditions. In response to actinomycin D, hydrogen peroxide (H2O2), or TNF-alpha, Jurkat T cells underwent typical apoptosis. Phospholipase D (PLD) activity in intact cells determined by phosphatidylbutanol generation was up-regulated by these agents. The PLD activation was in a time-dependent manner during apoptosis. It was also shown that the PLD activity measured by using exogenous substrate in the lysate from apoptotic cells was higher than that in the lysate from control untreated cells. The PLD activity in lysate from control untreated cells was stimulated by unsaturated fatty acids (UFA), but not by guanosine 5'-O-(3-thiotriphosphate). However, the PLD activity in the apoptotic cell lysate was no longer enhanced by the addition of oleate, suggesting that the increased PLD activity during apoptosis was attributed to the PLD of UFA-dependent type, but not the small G protein-dependent one. In fact, the release of free UFA was increased during apoptosis. The caspase inhibitors, z-DEVD and z-VAD, effectively suppressed PLD activation and apoptosis, but UFA release was unaffected. These results suggest the possibility that UFA-dependent type PLD may be implicated in apoptotic process in Jurkat T cells. This is the first demonstration that the PLD of UFA-dependent type would be involved in cellular responses.
巻・号 161(12)
ページ 6469-74
公開日 1998-12-15
PMID 9862669
MeSH Apoptosis / drug effects* Arachidonic Acid / metabolism Cell-Free System Dactinomycin / pharmacology* Enzyme Activation / drug effects Fatty Acids, Unsaturated / pharmacology Guanosine Triphosphate / pharmacology HL-60 Cells / drug effects HL-60 Cells / enzymology Humans Hydrogen Peroxide / pharmacology Isoenzymes / metabolism* Jurkat Cells / cytology Jurkat Cells / drug effects* Jurkat Cells / enzymology Membrane Lipids / metabolism Oleic Acid / metabolism Oleic Acid / pharmacology Phospholipase D / metabolism* T-Lymphocytes / cytology T-Lymphocytes / drug effects* T-Lymphocytes / enzymology Tumor Necrosis Factor-alpha / pharmacology
IF 4.886
引用数 72
WOS 分野 IMMUNOLOGY
リソース情報
ヒト・動物細胞 Jurkat(RCB0806)