Reference - Detail
| RRC ID | 39211 |
|---|---|
| Author | Takadera T, Ohyashiki T. |
| Title | Apoptotic cell death and CPP32-like activation induced by thapsigargin and their prevention by nerve growth factor in PC12 cells. |
| Journal | Biochim Biophys Acta |
| Abstract |
Thapsigargin, an endoplasmic reticular Ca2+-ATPase inhibitor, induced apoptotic cell death (chromatin condensation and DNA fragmentation) accompanied by the activation of CPP32-like protease, a member of the interleukin-1beta converting enzyme protease (ICE) family, but not the activation of ICE-like protease. Nerve growth factor (NGF) completely inhibited the cell death and CPP32-like activation induced by thapsigargin while Ac-Asp-Glu-Val-Asp-CHO, an inhibitor of CPP32-like protease, reduced the cell death. PD98059, a specific inhibitor of Map kinase kinase, did not reduce the protective effect of NGF on thapsigargin-induced cell death. These results suggest that calcium ion-induced apoptotic cell death was mediated by CPP32-like, but not ICE-like, protease and was regulated by a neurotrophic factor possibly, through the Map kinase cascade independent pathway. |
| Volume | 1401(1) |
| Pages | 63-71 |
| Published | 1998-1-2 |
| DOI | 10.1016/s0167-4889(97)00116-x |
| PII | S0167-4889(97)00116-X |
| PMID | 9459486 |
| MeSH | Animals Apoptosis / drug effects* Calcium / analysis Calcium-Transporting ATPases / antagonists & inhibitors* Caspase 1 Caspase 3 Caspases* Cycloheximide / pharmacology Cysteine Endopeptidases / metabolism* Cysteine Proteinase Inhibitors / pharmacology Enzyme Activation Enzyme Inhibitors / pharmacology Flavonoids / pharmacology L-Lactate Dehydrogenase / metabolism Mitogen-Activated Protein Kinase Kinases Nerve Growth Factors / pharmacology* Neurons Oligopeptides / pharmacology PC12 Cells Protein Kinase Inhibitors Protein Synthesis Inhibitors / pharmacology Rats Thapsigargin / pharmacology* |
| IF | 3.411 |
| Times Cited | 30 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Resource | |
| Human and Animal Cells | PC-12(RCB0009) |