| RRC ID |
39219
|
| 著者 |
Matsumura S, Hiranuma H, Deguchi A, Maeda T, Jikko A, Fuchihata H.
|
| タイトル |
Changes in phenotypic expression of osteoblasts after X irradiation.
|
| ジャーナル |
Radiat Res
|
| Abstract |
Changes in the phenotypic expression of osteoblasts after X irradiation were investigated. Osteoblast-like MC3T3-E1 cells at the actively proliferating, confluent and postproliferation stages were subjected to 10 Gy X irradiation. Irradiation at the confluent stage enhanced accumulation of type I collagen normalized to the DNA content. Irradiation at all stages down-regulated the expression of osteocalcin, but the levels of osteopontin and osteonectin mRNAs were unchanged from the control level. After irradiation at the later stages, the time-dependent increase in alkaline phosphatase activity per cell exceeded that in the control cells. The localization of alkaline phosphatase-positive cells was concordant with that of calcification. In addition, the quality of the calcium deposits was found to be similar to that in control cells as determined by energy dispersive spectrometry and the ratio of calcium to phosphorus, even if the cells were not exactly the same morphologically. The changes in phenotypic expression observed here are closely related to the enhancement of calcification observed in a previous study.
|
| 巻・号 |
149(5)
|
| ページ |
463-71
|
| 公開日 |
1998-5-1
|
| PMID |
9588357
|
| MeSH |
3T3 Cells
Alkaline Phosphatase / metabolism
Animals
Calcium Phosphates / metabolism
Dose-Response Relationship, Radiation
Extracellular Matrix Proteins / biosynthesis
Extracellular Matrix Proteins / genetics
Kinetics
Mice
Osteoblasts / enzymology
Osteoblasts / metabolism
Osteoblasts / radiation effects*
Phenotype
RNA, Messenger / genetics
RNA, Messenger / metabolism
|
| IF |
2.657
|
| 引用数 |
17
|
|
WOS 分野
|
RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
BIOLOGY
BIOPHYSICS
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
