| RRC ID |
39221
|
| Author |
Miura S, Hasumi K, Takayasu R, Sugimoto M, Endo A.
|
| Title |
Enhancement of low density lipoprotein binding to both low density lipoprotein receptor-positive and -negative cells by tetracycline antibiotics.
|
| Journal |
Lipids
|
| Abstract |
Some tetracycline (TC) antibiotics, including TC and anhydrotetracycline, have been found to enhance specific binding of low density lipoprotein (LDL) to both LDL receptor-positive and -negative cells at relatively higher concentrations. When incubated at 37 degrees C, the ability of LDL receptor-negative human fibroblasts to bind 125I-LDL was increased from < 2 to 45 ng/mg by 170 microM TC. In normal human fibroblasts and Hep G2 cells, 125I-LDL binding was elevated 1.4- to 2-fold by 113 microM TC. The 125I-LDL binding in the presence of TC was diminished by both heparin and EDTA. The enhancement by TC was not observed when 125I-LDL binding was assayed at 4 degrees C. TC enhanced LDL binding to paraformaldehyde-fixed Hep G2 cells, excluding LDL receptor induction in the mechanism. These results demonstrated that TC enhanced cellular LDL binding through a process not involving functional LDL receptors.
|
| Volume |
33(1)
|
| Pages |
33-8
|
| Published |
1998-1-1
|
| DOI |
10.1007/s11745-998-0177-y
|
| PMID |
9470171
|
| MeSH |
Anti-Bacterial Agents / pharmacology*
Cell Line
Cells / drug effects*
Cells / metabolism*
Cells, Cultured
Chelating Agents / pharmacology
Edetic Acid / pharmacology
Fibroblasts / drug effects
Fibroblasts / metabolism
Heparin / pharmacology
Humans
Lipoproteins, LDL / metabolism*
Macrophages / drug effects
Macrophages / metabolism
Membrane Proteins*
Receptors, Immunologic / metabolism
Receptors, LDL / analysis*
Receptors, LDL / metabolism
Receptors, Lipoprotein*
Receptors, Scavenger
Scavenger Receptors, Class B
Tetracyclines
|
| IF |
1.919
|
| Times Cited |
0
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
NUTRITION & DIETETICS
|
| Resource |
| Human and Animal Cells |
Hep G2(RCB0459) |
