RRC ID 39221
Author Miura S, Hasumi K, Takayasu R, Sugimoto M, Endo A.
Title Enhancement of low density lipoprotein binding to both low density lipoprotein receptor-positive and -negative cells by tetracycline antibiotics.
Journal Lipids
Abstract Some tetracycline (TC) antibiotics, including TC and anhydrotetracycline, have been found to enhance specific binding of low density lipoprotein (LDL) to both LDL receptor-positive and -negative cells at relatively higher concentrations. When incubated at 37 degrees C, the ability of LDL receptor-negative human fibroblasts to bind 125I-LDL was increased from < 2 to 45 ng/mg by 170 microM TC. In normal human fibroblasts and Hep G2 cells, 125I-LDL binding was elevated 1.4- to 2-fold by 113 microM TC. The 125I-LDL binding in the presence of TC was diminished by both heparin and EDTA. The enhancement by TC was not observed when 125I-LDL binding was assayed at 4 degrees C. TC enhanced LDL binding to paraformaldehyde-fixed Hep G2 cells, excluding LDL receptor induction in the mechanism. These results demonstrated that TC enhanced cellular LDL binding through a process not involving functional LDL receptors.
Volume 33(1)
Pages 33-8
Published 1998-1-1
DOI 10.1007/s11745-998-0177-y
PMID 9470171
MeSH Anti-Bacterial Agents / pharmacology* Cell Line Cells / drug effects* Cells / metabolism* Cells, Cultured Chelating Agents / pharmacology Edetic Acid / pharmacology Fibroblasts / drug effects Fibroblasts / metabolism Heparin / pharmacology Humans Lipoproteins, LDL / metabolism* Macrophages / drug effects Macrophages / metabolism Membrane Proteins* Receptors, Immunologic / metabolism Receptors, LDL / analysis* Receptors, LDL / metabolism Receptors, Lipoprotein* Receptors, Scavenger Scavenger Receptors, Class B Tetracyclines
IF 1.919
Times Cited 0
Human and Animal Cells Hep G2(RCB0459)