RRC ID 39222
著者 Kataoka H, Joh T, Kasugai K, Okayama N, Moriyama A, Asai K, Kato T.
タイトル Expression of mRNA for heregulin and its receptor, ErbB-3 and ErbB-4, in human upper gastrointestinal mucosa.
ジャーナル Life Sci
Abstract Expression of mRNA for heregulin (HRG), a member of the epidermal growth factor (EGF) family and its receptors, ErbB-3 and ErbB-4, were evaluated in human upper gastrointestinal (GI) mucosa. Multi-target reverse-transcriptase polymerase chain reaction (RT-PCR) analysis using capillary electrophoresis and laser-induced fluorescence allowed us to quantify the minute amounts of mRNA from one biopsy specimen with high sensitivity. HRG, ErbB-3 and ErbB-4 mRNA were detected in esophagus, stomach and duodenum and the highest expression was found in duodenum. In gastric cancer, mRNA for ErbB-4 was significantly overexpressed. Immunoreactivity of ErbB-4 in carcinoma cell membrane was also confirmed. These findings suggest that HRG and its receptors, ErbB-3 and ErbB-4 may be physiologically significant in the human upper GI mucosa, especially in duodenum, and that ErbB-4 may contribute to the growth of gastric cancer.
巻・号 63(7)
ページ 553-64
公開日 1998-1-1
DOI 10.1016/s0024-3205(98)00306-3
PII S0024320598003063
PMID 9718081
MeSH ErbB Receptors / genetics* Glycoproteins / genetics* Humans Intestinal Mucosa / metabolism* Nerve Growth Factors / genetics* Neuregulins Oligonucleotides, Antisense / metabolism Proto-Oncogene Proteins / genetics* RNA, Messenger / metabolism* Receptor, ErbB-3 Receptor, ErbB-4 Tumor Cells, Cultured
IF 3.647
引用数 41
WOS 分野 PHARMACOLOGY & PHARMACY MEDICINE, RESEARCH & EXPERIMENTAL
リソース情報
ヒト・動物細胞 MKN45(RCB1001) MKN74(RCB1002)