RRC ID |
39222
|
著者 |
Kataoka H, Joh T, Kasugai K, Okayama N, Moriyama A, Asai K, Kato T.
|
タイトル |
Expression of mRNA for heregulin and its receptor, ErbB-3 and ErbB-4, in human upper gastrointestinal mucosa.
|
ジャーナル |
Life Sci
|
Abstract |
Expression of mRNA for heregulin (HRG), a member of the epidermal growth factor (EGF) family and its receptors, ErbB-3 and ErbB-4, were evaluated in human upper gastrointestinal (GI) mucosa. Multi-target reverse-transcriptase polymerase chain reaction (RT-PCR) analysis using capillary electrophoresis and laser-induced fluorescence allowed us to quantify the minute amounts of mRNA from one biopsy specimen with high sensitivity. HRG, ErbB-3 and ErbB-4 mRNA were detected in esophagus, stomach and duodenum and the highest expression was found in duodenum. In gastric cancer, mRNA for ErbB-4 was significantly overexpressed. Immunoreactivity of ErbB-4 in carcinoma cell membrane was also confirmed. These findings suggest that HRG and its receptors, ErbB-3 and ErbB-4 may be physiologically significant in the human upper GI mucosa, especially in duodenum, and that ErbB-4 may contribute to the growth of gastric cancer.
|
巻・号 |
63(7)
|
ページ |
553-64
|
公開日 |
1998-1-1
|
DOI |
10.1016/s0024-3205(98)00306-3
|
PII |
S0024320598003063
|
PMID |
9718081
|
MeSH |
ErbB Receptors / genetics*
Glycoproteins / genetics*
Humans
Intestinal Mucosa / metabolism*
Nerve Growth Factors / genetics*
Neuregulins
Oligonucleotides, Antisense / metabolism
Proto-Oncogene Proteins / genetics*
RNA, Messenger / metabolism*
Receptor, ErbB-3
Receptor, ErbB-4
Tumor Cells, Cultured
|
IF |
3.647
|
引用数 |
41
|
WOS 分野
|
PHARMACOLOGY & PHARMACY
MEDICINE, RESEARCH & EXPERIMENTAL
|
リソース情報 |
ヒト・動物細胞 |
MKN45(RCB1001)
MKN74(RCB1002) |