論文 - 詳細
| RRC ID | 39243 |
|---|---|
| 著者 | Taniguchi A, Higai K, Hasegawa Y, Utsumi K, Matsumoto K. |
| タイトル | Differentiation elicits negative regulation of human beta-galactoside alpha2,6-sialyltransferase at the mRNA level in the HL-60 cell line. |
| ジャーナル | FEBS Lett |
| Abstract |
We studied the regulation of the beta-galactoside alpha2,6-sialyltransferase (hST6Gal I) gene during HL-60 cell differentiation induced with dimethyl sulfoxide (DMSO), all transretinoic acid (ATRA), and phorbol myristate acetate (PMA). During HL-60 cell line differentiation, cell surface levels of alpha2,6-sialic acids expression decreased, as measured by flow cytometric analysis using Sambucus nigra agglutinin (SNA). Activities of hST6Gal I and levels of hST6Gal I mRNA dramatically decreased after 1 day of stimulation. Using reverse transcription polymerase chain reaction (PT-PCR), we found the major hST6Gal I mRNA isoform in HL-60 cells contains 5'-untranslated exons Y and Z. These results suggest that the expression of cell surface alpha2,6-sialic acids is controlled at the mRNA level, which is regulated by a promoter located 5'-upstream of exon Y. |
| 巻・号 | 441(2) |
| ページ | 191-4 |
| 公開日 | 1998-12-18 |
| DOI | 10.1016/s0014-5793(98)01548-8 |
| PII | S0014-5793(98)01548-8 |
| PMID | 9883882 |
| MeSH | Base Sequence Cell Differentiation / drug effects Cell Differentiation / genetics* Cell Membrane / metabolism DNA Primers Dimethyl Sulfoxide / pharmacology Exons Gene Expression Regulation, Enzymologic / drug effects* HL-60 Cells Humans Polymerase Chain Reaction Promoter Regions, Genetic RNA, Messenger / genetics* Sialic Acids / metabolism Sialyltransferases / genetics* Tetradecanoylphorbol Acetate / pharmacology Tretinoin / pharmacology beta-D-Galactoside alpha 2-6-Sialyltransferase |
| IF | 3.057 |
| 引用数 | 22 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | Hep G2(RCB0459) HuH-7(RCB1366) |