論文 - 詳細
| RRC ID | 39244 |
|---|---|
| 著者 | Kakeya H, Onose R, Liu PC, Onozawa C, Matsumura F, Osada H. |
| タイトル | Inhibition of cyclin D1 expression and phosphorylation of retinoblastoma protein by phosmidosine, a nucleotide antibiotic. |
| ジャーナル | Cancer Res |
| Abstract |
In this report, we studied the effect of phosmidosine, a proline-containing nucleotide on the serum-induced cell cycle progression in human lung fibroblast WI-38 cells. Phosmidosine suppressed S-phase entry and arrested cell cycle progression at the G1 phase. In serum-stimulated cells, phosmidosine did not affect the activation of the mitogen-activated protein kinase cascade. However, phosmidosine inhibited hyperphosphorylation of retinoblastoma (RB) protein by RB-kinases such as cyclin-dependent kinase 4 and cyclin-dependent kinase 2, probably as a result of the inhibition of cyclin D1 expression. Furthermore, in tsFT210 cells, a temperature-sensitive cdc2 mutant isolated from the mouse mammary carcinoma cell line FM3A, phosmidosine, irreversibly inhibited the cell cycle progression at G1 without affecting the G2 to M transition. Phosmidosine acts at an earlier point in G1 compared with mimosine or aphidicolin, well-known cell cycle blockers at the G1-S boundary. Taken together, phosmidosine arrested cells at a specific point between the start point and restriction point in G1 and is a useful drug that may contribute to the understanding of the regulatory mechanisms of G1 progression. |
| 巻・号 | 58(4) |
| ページ | 704-10 |
| 公開日 | 1998-2-15 |
| PMID | 9485024 |
| MeSH | Animals Antibiotics, Antineoplastic / pharmacology* Calcium-Calmodulin-Dependent Protein Kinases / metabolism Cell Cycle / drug effects Cyclin D1 / metabolism* Enzyme Activation Humans Mice Mitogen-Activated Protein Kinase 3 Mitogen-Activated Protein Kinases* Phosphorylation Purine Nucleotides / pharmacology Retinoblastoma Protein / metabolism* Tumor Cells, Cultured |
| IF | 9.727 |
| 引用数 | 22 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | WI-38 |