Reference - Detail
| RRC ID | 39261 |
|---|---|
| Author | Ishiguro H, Yamada K, Ichino N, Nagatsu T. |
| Title | Identification and characterization of a novel phorbol ester-responsive DNA sequence in the 5'-flanking region of the human dopamine beta-hydroxylase gene. |
| Journal | J Biol Chem |
| Abstract |
The phorbol ester, 12-O-tetradecanoylphorbol-13-acetate (TPA), enhances transcription of many eukaryotic genes, including that for dopamine beta-hydroxylase (DBH). In the present study, we report identification and characterization of a novel sequence motif residing in the 5'-flanking region of the human DBH gene, which mediates transcriptional induction by TPA. Deletional analyses indicated the promoter region between -223 and -187 base pairs to be critical. Whereas this region does not contain any putative regulatory motifs with significant sequence homology to the AP-1 motif, extensive deletional and site-directed mutational analyses indicated that a sequence between -210 and -199 base pairs, 5'-ATCCGCCTGTCT-3', may represent a novel TPA-response element (TRE). In addition, alteration of the YY1-binding site decreased TPA-mediated induction of the DBH promoter activity, suggesting that contiguous cis-regulatory element(s) cooperate with this novel sequence motif. Furthermore, insertional mutation analyses between the YY1-binding site and the cyclic AMP-responsive element indicated that the stereospecificity of these motifs is important for intact transcriptional induction by TPA. Taken together, these data suggest that transcriptional up-regulation of the human DBH gene in response to TPA requires coordination of a novel TRE (human DBH TRE, hDTRE), cyclic AMP-responsive element, and the YY1-binding site. |
| Volume | 273(34) |
| Pages | 21941-9 |
| Published | 1998-8-21 |
| DOI | 10.1074/jbc.273.34.21941 |
| PII | S0021-9258(18)48869-2 |
| PMID | 9705334 |
| MeSH | Base Sequence Binding Sites Cyclic AMP Response Element-Binding Protein / metabolism DNA / chemistry DNA / drug effects DNA / metabolism* DNA-Binding Proteins / metabolism Dopamine beta-Hydroxylase / genetics* Erythroid-Specific DNA-Binding Factors Humans Molecular Sequence Data Mutagenesis, Site-Directed Promoter Regions, Genetic Structure-Activity Relationship Tetradecanoylphorbol Acetate / pharmacology* Transcription Factor AP-1 / metabolism Transcription Factors / metabolism Transcription, Genetic / drug effects* Up-Regulation YY1 Transcription Factor |
| IF | 4.238 |
| Times Cited | 11 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | SK-N-SH(RCB0426) |