Reference - Detail
| RRC ID | 39263 |
|---|---|
| Author | Yoshida A, Minowa MT, Takamatsu S, Hara T, Ikenaga H, Takeuchi M. |
| Title | A novel second isoenzyme of the human UDP-N-acetylglucosamine:alpha1,3-D-mannoside beta1,4-N-acetylglucosaminyltransferase family: cDNA cloning, expression, and chromosomal assignment. |
| Journal | Glycoconj J |
| Abstract |
We isolated a novel cDNA encoding a second isoenzyme of UDP-N-acetylglucosamine:alpha1,3-D-mannoside beta1,4-N-acetylglucosaminyltransferase (GnT-IV; EC 2.4.1.145). The nucleotide and deduced amino acid sequences of the cDNA were homologous to those of the previously cloned human GnT-IV cDNA (63% and 62% identity, respectively). The new cDNA is also confirmed to express GnT-IV activity, suggesting that two isoenzymes of human GnT-IV exist. Although genomic Southern analysis suggested that both genes exist in many mammalian species and the chicken, northern analysis revealed that both genes are expressed in different ways in human tissues. This is the first report concerning the gene family of an N-acetylglucosaminyltransferase in mammals. |
| Volume | 15(12) |
| Pages | 1115-23 |
| Published | 1998-12-1 |
| DOI | 10.1023/a:1006951519522 |
| PMID | 10372966 |
| MeSH | Amino Acid Sequence Animals Base Sequence Blotting, Northern Blotting, Southern COS Cells Chromosome Mapping Chromosomes, Human, Pair 5* Cloning, Molecular DNA Primers DNA, Complementary Humans Isoenzymes / genetics* Molecular Sequence Data N-Acetylglucosaminyltransferases / genetics* Sequence Homology, Amino Acid Tumor Cells, Cultured |
| IF | 2.197 |
| Times Cited | 27 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 9 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | COS-7(RCB0539) |