RRC ID 39272
Author Iwabuchi K, Li B, Massa HF, Trask BJ, Date T, Fields S.
Title Stimulation of p53-mediated transcriptional activation by the p53-binding proteins, 53BP1 and 53BP2.
Journal J. Biol. Chem.
Abstract p53 is a tumor suppressor protein that controls cell proliferation by regulating the expression of growth control genes. In a previous study, we identified two proteins, 53BP1 and 53BP2, that are able to bind to wild type but not to mutant p53 via the DNA-binding domain of p53. We isolated cDNAs expressing a full-length human 53BP1 clone, which predicts a protein of 1972 residues that can be detected in the H358 human lung carcinoma cell line. The 53BP1 and 53BP2 genes were mapped to chromosomes 15q15-21 and 1q41-42, respectively. Immunofluorescence studies showed three types of staining patterns for 53BP1 as follows: both cytoplasmic and nuclear, homogeneous nuclear, and a nuclear dot pattern. In contrast, 53BP2 localized exclusively to the cytoplasm, and this pattern did not change upon coexpression of wild type p53. Although our previous study revealed that p53 is not able to bind simultaneously to either 53BP1 or 53BP2 and to DNA carrying a consensus binding site, both 53BP1 and 53BP2 enhanced p53-mediated transcriptional activation and induced the expression of a p53-dependent protein, suggesting that these proteins might function in signal transduction pathways to promote p53 activity.
Volume 273(40)
Pages 26061-8
Published 1998-10-2
DOI 10.1074/jbc.273.40.26061
PMID 9748285
MeSH Amino Acid Sequence Apoptosis Regulatory Proteins Carrier Proteins / chemistry* Carrier Proteins / physiology* Chromosome Mapping Chromosomes, Human, Pair 1 / genetics Chromosomes, Human, Pair 15 / genetics Cloning, Molecular DNA-Binding Proteins / metabolism Fluorescent Antibody Technique Genes, ras / genetics Humans Intracellular Signaling Peptides and Proteins* Molecular Sequence Data Phosphoproteins* Sequence Analysis, DNA Suppression, Genetic / genetics Transcriptional Activation / genetics* Transfection / genetics Tumor Cells, Cultured Tumor Suppressor Protein p53 / metabolism* Tumor Suppressor p53-Binding Protein 1
IF 4.106
Times Cited 150
Human and Animal Cells