論文 - 詳細
| RRC ID | 39273 |
|---|---|
| 著者 | Yanaka N, Akatsuka H, Kawai E, Omori K. |
| タイトル | 1,25-Dihydroxyvitamin D3 upregulates natriuretic peptide receptor-C expression in mouse osteoblasts. |
| ジャーナル | Am J Physiol |
| Abstract |
1,25-Dihydroxyvitamin D3 [1,25(OH)2D3], a key regulator of mineral metabolism, regulates expression of several genes related to bone formation. The present study examined the 1,25(OH)2D3-mediated regulation of natriuretic peptide receptor-C (NPR-C) expression in osteoblasts. 1,25(OH)2D3 treatment significantly increased NPR-C-dependent atrial natriuretic peptide-binding activity and synthesis of the NPR-C protein in mouse osteoblastic cells in a cell-specific manner. Western blot analysis also demonstrated that 1, 25(OH)2D3 upregulated expression of NPR-C protein in slow kinetics. Next, Northern blot analysis revealed a significant increase in the steady-state NPR-C mRNA level by 1,25(OH)2D3. Sequence analysis of the 9 kb of the 5'-flanking region of the mouse NPR-C gene revealed an absence of consensus vitamin D-response elements, and promoter analysis using osteoblastic cells stably transfected with mouse NPR-C promoter-reporter constructs showed a slight increase of promoter activity with 1,25(OH)2D3 treatment. In addition, a nuclear run-on assay exhibited that the transcriptional rate of the NPR-C gene was unchanged by 1,25(OH)2D3, whereas that of the osteopontin gene was increased. Evaluation of NPR-C mRNA half-life demonstrated that 1,25(OH)2D3 significantly increased the NPR-C mRNA stability in osteoblastic cells. 1,25(OH)2D3 attenuated intracellular cGMP production in osteoblastic cells stimulated by C-type natriuretic peptide (CNP) without a significant change of the natriuretic peptide receptor-B mRNA level, suggesting enhancement of the clearance of exogenously added CNP via NPR-C. Furthermore, NPR-C and osteopontin mRNAs in mouse calvariae were significantly increased by administration of 1,25(OH)2D3, and immunohistological analysis demonstrated that NPR-C is actually and strongly expressed in mouse periosteal fibroblasts. These findings suggest that 1,25(OH)2D3 can play a critical role for determination of the natriuretic peptide availability in bones by regulation of NPR-C expression through stabilizing its mRNA. |
| 巻・号 | 275(6) |
| ページ | E965-73 |
| 公開日 | 1998-12-1 |
| DOI | 10.1152/ajpendo.1998.275.6.E965 |
| PMID | 9843738 |
| MeSH | Animals Calcitriol / pharmacology* Cells, Cultured Guanylate Cyclase / drug effects* Guanylate Cyclase / genetics Guanylate Cyclase / metabolism* Mice Mice, Inbred ICR Molecular Sequence Data Natriuretic Peptide, C-Type / pharmacology Osteoblasts / drug effects* Osteoblasts / metabolism* RNA, Messenger / metabolism Rats Rats, Wistar Receptors, Atrial Natriuretic Factor / drug effects* Receptors, Atrial Natriuretic Factor / genetics Receptors, Atrial Natriuretic Factor / metabolism* Up-Regulation / physiology* |
| IF | 3.485 |
| WOS 分野 | PHYSIOLOGY ENDOCRINOLOGY & METABOLISM |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 10T1/2(RCB0247) |