RRC ID 39280
著者 Matsuya M, Sasaki H, Aoto H, Mitaka T, Nagura K, Ohba T, Ishino M, Takahashi S, Suzuki R, Sasaki T.
タイトル Cell adhesion kinase beta forms a complex with a new member, Hic-5, of proteins localized at focal adhesions.
ジャーナル J Biol Chem
Abstract Cell adhesion kinase beta (CAKbeta/PYK2) is the second protein-tyrosine kinase of the focal adhesion kinase subfamily. We identified a cDNA that encodes a CAKbeta-binding protein. This cDNA clone encodes the human homologue of Hic-5, the cDNA of which was cloned in 1994 as transforming growth factor beta1- and hydrogen peroxide-inducible mRNA. We found that Hic-5 exclusively localized at focal adhesions in a rat fibroblast line, WFB. This localization of Hic-5 was confirmed in WFB cells expressing Myc-tagged Hic-5. The amino acid sequence of Hic-5 is highly similar to that of paxillin in the four LD motifs as well as in the four contiguous LIM domains. The Hic-5 N-terminal domain directly associated in vitro with the extreme C-terminal region (residue 801 to the end) of CAKbeta. CAKbeta was coimmunoprecipitated with Hic-5 from the WFB cell lysate. The coimmunoprecipitation of CAKbeta with Hic-5 was markedly inhibited by the addition of the extreme C-terminal region of CAKbeta. Coimmunoprecipitation of Hic-5 with CAKbeta, which was shown in COS-7 cells doubly transfected with cDNA constructs of CAKbeta and Myc-tagged Hic-5, was lost when the CAKbeta amino acid residues 741-903 were deleted. Hic-5 was tyrosine-phosphorylated in Src-transformed 3Y1 cells and in cells treated with pervanadate. Hic-5 associated with CAKbeta was selectively tyrosine-phosphorylated in WFB cells exposed to hypertonic osmotic stress. These results indicate that Hic-5 is a paxillin-related component of focal adhesions and binds to CAKbeta, implying possible involvement of Hic-5 in the downstream signaling of CAKbeta.
巻・号 273(2)
ページ 1003-14
公開日 1998-1-9
DOI 10.1074/jbc.273.2.1003
PII S0021-9258(18)39569-3
PMID 9422762
MeSH Amino Acid Sequence Animals COS Cells Cell Line Child, Preschool Cloning, Molecular Cytoskeletal Proteins / chemistry DNA, Complementary DNA-Binding Proteins / chemistry DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Female Focal Adhesion Kinase 2 Glutathione Transferase / genetics Humans Intracellular Signaling Peptides and Proteins LIM Domain Proteins Lysophospholipids / pharmacology Molecular Sequence Data Oxidative Stress Paxillin Phosphoproteins / chemistry Precipitin Tests Protein-Tyrosine Kinases / genetics Protein-Tyrosine Kinases / metabolism* Rats Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Sequence Homology, Amino Acid
IF 4.238
引用数 111
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞