Reference - Detail
RRC ID | 39288 |
---|---|
Author | Kitani T, Ishida A, Okuno S, Takeuchi M, Kameshita I, Fujisawa H. |
Title | Molecular cloning of Ca2+/calmodulin-dependent protein kinase phosphatase. |
Journal | J Biochem |
Abstract |
Calmodulin-dependent protein kinase (CaM-kinase) phosphatase dephosphorylates and concomitantly deactivates CaM-kinase II activated upon autophosphorylation, and CaM-kinases IV and I activated upon phosphorylation by CaM-kinase kinase [Ishida, I., Okuno, S., Kitani, T., Kameshita, I., and Fujisawa, H. (1998) Biochem. Biophys. Res. Commun. 253, 159-163], suggesting that CaM-kinase phosphatase plays important roles in the function of Ca2+ in the cell, because the three multifunctional CaM-kinases (CaM-kinases I, II, and IV) are thought to be the key enzymes in the Ca2+-signaling system. In the present study, cDNA for CaM-kinase phosphatase was cloned from a rat brain cDNA library. The coded protein consisted of 450 amino acids with a molecular weight of 49, 165. Western blot analysis showed the ubiquitous tissue distribution of CaM-kinase phosphatase. Immunocytochemical analysis revealed that CaM-kinase phosphatase is evenly distributed outside the nucleus in a cell. |
Volume | 125(6) |
Pages | 1022-8 |
Published | 1999-6-1 |
DOI | 10.1093/oxfordjournals.jbchem.a022381 |
PMID | 10348902 |
MeSH | Amino Acid Sequence Animals Base Sequence Brain / enzymology Cloning, Molecular DNA, Complementary / genetics Escherichia coli / genetics Humans Mitogen-Activated Protein Kinases / metabolism Molecular Sequence Data Molecular Weight Protein Tyrosine Phosphatases / chemistry Protein Tyrosine Phosphatases / genetics* Protein Tyrosine Phosphatases / metabolism Rabbits Rats Recombinant Proteins / chemistry Recombinant Proteins / genetics Recombinant Proteins / metabolism Sequence Homology, Amino Acid Species Specificity Subcellular Fractions / enzymology Tissue Distribution |
IF | 2.476 |
Times Cited | 50 |
WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
Resource | |
Human and Animal Cells |